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dc.contributor.authorGómez-Redondo, Isabel
dc.contributor.authorPlanells, Benjamín
dc.contributor.authorCánovas, Sebastián
dc.contributor.authorIvanova, Elena
dc.contributor.authorKelsey, Gavin
dc.contributor.authorGutiérrez-Adán, Alfonso
dc.date.accessioned2021-02-04T16:25:35Z
dc.date.available2021-02-04T16:25:35Z
dc.date.issued2021-02-03
dc.date.submitted2020-07-08
dc.identifier.issn1868-7075
dc.identifier.others13148-021-01003-x
dc.identifier.other1003
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/317161
dc.description.abstractBACKGROUND: Prior work in mice has shown that some retrotransposed elements remain substantially methylated during DNA methylation reprogramming of germ cells. In the pig, however, information about this process is scarce. The present study was designed to examine the methylation profiles of porcine germ cells during the time course of epigenetic reprogramming. RESULTS: Sows were artificially inseminated, and their fetuses were collected 28, 32, 36, 39, and 42 days later. At each time point, genital ridges were dissected from the mesonephros and germ cells were isolated through magnetic-activated cell sorting using an anti-SSEA-1 antibody, and recovered germ cells were subjected to whole-genome bisulphite sequencing. Methylation levels were quantified using SeqMonk software by performing an unbiased analysis, and persistently methylated regions (PMRs) in each sex were determined to extract those regions showing 50% or more methylation. Most genomic elements underwent a dramatic loss of methylation from day 28 to day 36, when the lowest levels were shown. By day 42, there was evidence for the initiation of genomic re-methylation. We identified a total of 1456 and 1122 PMRs in male and female germ cells, respectively, and large numbers of transposable elements (SINEs, LINEs, and LTRs) were found to be located within these PMRs. Twenty-one percent of the introns located in these PMRs were found to be the first introns of a gene, suggesting their regulatory role in the expression of these genes. Interestingly, most of the identified PMRs were demethylated at the blastocyst stage. CONCLUSIONS: Our findings indicate that methylation reprogramming in pig germ cells follows the general dynamics shown in mice and human, unveiling genomic elements that behave differently between male and female germ cells.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectResearch
dc.subjectReproductive and Transgenerational Epigenetics
dc.subjectEmbryo
dc.subjectPrimordial germ cells
dc.subjectMethylation reprogramming
dc.subjectWhole-genome bisulphite sequencing
dc.titleGenome-wide DNA methylation dynamics during epigenetic reprogramming in the porcine germline.
dc.typeArticle
dc.date.updated2021-02-04T16:25:35Z
prism.issueIdentifier1
prism.publicationNameClin Epigenetics
prism.volume13
dc.identifier.doi10.17863/CAM.64272
dcterms.dateAccepted2021-01-04
rioxxterms.versionofrecord10.1186/s13148-021-01003-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidGómez-Redondo, Isabel [0000-0002-9319-7580]
dc.identifier.eissn1868-7083
pubs.funder-project-idMinisterio de Ciencia e Innovación (RTI2018-093548-BI00, BES-2016- 077794)
pubs.funder-project-idMarie Skłodowska-Curie ITN European Joint Doctorate in Biology and Technology of Reproductive Health (REP-BIOTECH 675526)
cam.issuedOnline2021-02-03


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)