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dc.contributor.authorBlackwell, Jenefer M
dc.contributor.authorFakiola, Michaela
dc.contributor.authorCastellucci, Léa C
dc.date.accessioned2021-02-12T17:30:35Z
dc.date.available2021-02-12T17:30:35Z
dc.date.issued2020-06
dc.date.submitted2019-12-05
dc.identifier.issn0340-6717
dc.identifier.others00439-020-02130-w
dc.identifier.other2130
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/317563
dc.description.abstractIdentifying genetic risk factors for parasitic infections such as the leishmaniases could provide important leads for improved therapies and vaccines. Until recently most genetic studies of human leishmaniasis were underpowered and/or not replicated. Here, we focus on recent genome-wide association studies of visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). For VL, analysis across 2287 cases and 2692 controls from three cohorts identified a single major peak of genome-wide significance (Pcombined = 2.76 × 10-17) at HLA-DRB1-HLA-DQA1. HLA-DRB1*1501 and DRB1*1404/DRB1*1301 were the most significant protective versus risk alleles, respectively, with specific residues at amino acid positions 11 and 13 unique to protective alleles. Epitope-binding studies showed higher frequency of basic AAs in DRB1*1404-/*1301-specific epitopes compared to hydrophobic and polar AAs in DRB1*1501-specific epitopes at anchor residues P4 and P6 which interact with residues at DRB1 positions 11 and 13. For CL, genome-wide significance was not achieved in combined analysis of 2066 cases and 2046 controls across 2 cohorts. Rather, multiple top hits at P < 5 × 10-5 were observed, amongst which IFNG-AS1 was of specific interest as a non-coding anti-sense RNA known to influence responses to pathogens by increasing IFN-γ secretion. Association at LAMP3 encoding dendritic cell lysosomal associated membrane protein 3 was also interesting. LAMP3 increases markedly upon activation of dendritic cells, localizing to the MHC Class II compartment immediately prior to translocation of Class II to the cell surface. Together these GWAS results provide firm confirmation for the importance of antigen presentation and the regulation of IFNγ in determining the outcome of Leishmania infections.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectReview
dc.subjectHuman genetics of infectious diseases
dc.titleHuman genetics of leishmania infections.
dc.typeArticle
dc.date.updated2021-02-12T17:30:34Z
prism.endingPage819
prism.issueIdentifier6-7
prism.publicationNameHum Genet
prism.startingPage813
prism.volume139
dc.identifier.doi10.17863/CAM.64676
dcterms.dateAccepted2020-02-02
rioxxterms.versionofrecord10.1007/s00439-020-02130-w
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidBlackwell, Jenefer M [0000-0002-0784-7277]
dc.identifier.eissn1432-1203
cam.issuedOnline2020-02-13


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)