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dc.contributor.authorGordon, Anna
dc.contributor.authorMcCartney, Curt
dc.contributor.authorKnox, Ron E
dc.contributor.authorEreful, Nelzo
dc.contributor.authorHiebert, Colin W
dc.contributor.authorKonkin, David J
dc.contributor.authorHsueh, Ya-Chih
dc.contributor.authorBhadauria, Vijai
dc.contributor.authorSgroi, Mara
dc.contributor.authorO'Sullivan, Donal M
dc.contributor.authorHadley, Caroline
dc.contributor.authorBoyd, Lesley A
dc.contributor.authorMenzies, Jim G
dc.date.accessioned2021-02-13T16:19:13Z
dc.date.available2021-02-13T16:19:13Z
dc.date.issued2020-06
dc.date.submitted2019-11-13
dc.identifier.issn0040-5752
dc.identifier.others00122-020-03561-9
dc.identifier.other3561
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/317610
dc.descriptionFunder: Canadian Seed Growers' Association; doi: http://dx.doi.org/10.13039/501100000071
dc.descriptionFunder: National Research Council of Canada: Canadian Wheat Improvement program
dc.description.abstractKEY MESSAGE: Four QTL for ergot resistance (causal pathogen Claviceps purpurea) have been identified in the durum wheat cultivar Greenshank. Claviceps purpurea is a pathogen of grasses that infects flowers, replacing the seed with an ergot sclerotium. Ergot presents a significant problem to rye, barley and wheat, in particular hybrid seed production systems. In addition, there is evidence that the highly toxic alkaloids that accumulate within sclerotia can cross-contaminate otherwise healthy grain. Host resistance to C. purpurea is rare, few resistance loci having been identified. In this study, four ergot resistance loci are located on chromosomes 1B, 2A, 5A and 5B in the durum wheat cv. Greenshank. Ergot resistance was assessed through analysis of phenotypes associated with C. purpurea infection, namely the number of inoculated flowers that produced sclerotia, or resulted in ovary death but no sclerotia, the levels of honeydew produced, total sclerotia weight and average sclerotia weight and size per spike. Ergot testing was undertaken in Canada and the UK. A major effect QTL, QCp.aafc.DH-2A, was detected in both the Canadian and UK experiments and had a significant effect on honeydew production levels. QCp.aafc.DH-5B had the biggest influence on total sclerotia weight per spike. QCp.aafc.DH-1B was only detected in the Canadian experiments and QCp.aafc.DH-5A in the UK experiment. An RNASeq analysis, undertaken to identify wheat differentially expressed genes associated with different combinations of the four ergot resistance QTL, revealed a disproportionate number of DEGs locating to the QCp.aafc.DH-1B, QCp.aafc.DH-2A and QCp.aafc.DH-5B QTL intervals.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectOriginal Article
dc.titleGenetic and transcriptional dissection of resistance to Claviceps purpurea in the durum wheat cultivar Greenshank.
dc.typeArticle
dc.date.updated2021-02-13T16:19:13Z
prism.endingPage1886
prism.issueIdentifier6
prism.publicationNameTheor Appl Genet
prism.startingPage1873
prism.volume133
dc.identifier.doi10.17863/CAM.64724
dcterms.dateAccepted2020-01-29
rioxxterms.versionofrecord10.1007/s00122-020-03561-9
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidGordon, Anna [0000-0003-3837-0237]
dc.contributor.orcidMenzies, Jim G [0000-0002-5591-8957]
dc.identifier.eissn1432-2242
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/G020418/1)
cam.issuedOnline2020-02-14


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)