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dc.contributor.authorVelazquez-Villarreal, Enrique I.
dc.contributor.authorMaheshwari, Shamoni
dc.contributor.authorSorenson, Jon
dc.contributor.authorFiddes, Ian T.
dc.contributor.authorKumar, Vijay
dc.contributor.authorYin, Yifeng
dc.contributor.authorWebb, Michelle G.
dc.contributor.authorCatalanotti, Claudia
dc.contributor.authorGrigorova, Mira
dc.contributor.authorEdwards, Paul A.
dc.contributor.authorCarpten, John D.
dc.contributor.authorCraig, David W.
dc.date.accessioned2021-02-17T16:39:03Z
dc.date.available2021-02-17T16:39:03Z
dc.date.issued2020-06-25
dc.date.submitted2019-09-26
dc.identifier.others42003-020-1044-8
dc.identifier.other1044
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/317793
dc.description.abstractAbstract: We performed shallow single-cell sequencing of genomic DNA across 1475 cells from a cell-line, COLO829, to resolve overall complexity and clonality. This melanoma tumor-line has been previously characterized by multiple technologies and is a benchmark for evaluating somatic alterations. In some of these studies, COLO829 has shown conflicting and/or indeterminate copy number and, thus, single-cell sequencing provides a tool for gaining insight. Following shallow single-cell sequencing, we first identified at least four major sub-clones by discriminant analysis of principal components of single-cell copy number data. Based on clustering, break-point and loss of heterozygosity analysis of aggregated data from sub-clones, we identified distinct hallmark events that were validated within bulk sequencing and spectral karyotyping. In summary, COLO829 exhibits a classical Dutrillaux’s monosomic/trisomic pattern of karyotype evolution with endoreduplication, where consistent sub-clones emerge from the loss/gain of abnormal chromosomes. Overall, our results demonstrate how shallow copy number profiling can uncover hidden biological insights.
dc.languageen
dc.publisherNature Publishing Group UK
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectArticle
dc.subject/631/67/2329
dc.subject/631/208/68
dc.subject/631/208/69
dc.subject/631/67/68
dc.subject/631/67/69
dc.subject/45
dc.subject/45/23
dc.subjectarticle
dc.titleSingle-cell sequencing of genomic DNA resolves sub-clonal heterogeneity in a melanoma cell line
dc.typeArticle
dc.date.updated2021-02-17T16:39:02Z
prism.issueIdentifier1
prism.publicationNameCommunications Biology
prism.volume3
dc.identifier.doi10.17863/CAM.64908
dcterms.dateAccepted2020-05-29
rioxxterms.versionofrecord10.1038/s42003-020-1044-8
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidEdwards, Paul A. [0000-0002-4789-3374]
dc.contributor.orcidCarpten, John D. [0000-0002-6862-2821]
dc.identifier.eissn2399-3642


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)