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In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture.

Published version
Peer-reviewed

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Authors

Richardson, Simon E 
Ghazanfari, Roshanak 
Chhetri, Jyoti 
Enver, Tariq 
Böiers, Charlotta 

Abstract

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).

Description

Keywords

Cell Differentiation, Cell culture, Stem Cells, Animals, B-Lymphocytes, Cell Line, Cell Separation, Coculture Techniques, Humans, Leukemia, Lymphoid, Mice, Pluripotent Stem Cells

Journal Title

STAR Protoc

Conference Name

Journal ISSN

2666-1667
2666-1667

Volume Title

2

Publisher

Elsevier BV
Sponsorship
Wellcome Trust (203151/Z/16/Z)
Cancer Research UK (C67279/A27957)
Wellcome Trust, Cancer Research UK, Swedish Research Council, Swedish Childhood Cancer Foundation