In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Richardson, Simon E
Ghazanfari, Roshanak
Chhetri, Jyoti
Enver, Tariq
Böiers, Charlotta
Abstract
In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).
Description
Keywords
Cell Differentiation, Cell culture, Stem Cells, Animals, B-Lymphocytes, Cell Line, Cell Separation, Coculture Techniques, Humans, Leukemia, Lymphoid, Mice, Pluripotent Stem Cells
Journal Title
STAR Protoc
Conference Name
Journal ISSN
2666-1667
2666-1667
2666-1667
Volume Title
2
Publisher
Elsevier BV
Publisher DOI
Sponsorship
Wellcome Trust (203151/Z/16/Z)
Cancer Research UK (C67279/A27957)
Cancer Research UK (C67279/A27957)
Wellcome Trust, Cancer Research UK, Swedish Research Council, Swedish Childhood Cancer Foundation