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Research data in support of: A modular, dynamic, DNA-based platform for regulating cargo distribution and transport between lipid domains


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Type

Dataset

Change log

Authors

Rubio Sanchez, Roger  ORCID logo  https://orcid.org/0000-0001-5574-5809
Eizagirre Barker, Simone 
Cicuta, Pietro 
Di Michele, Lorenzo 

Description

Confocal micrographs of phase-separated Giant Unilamellar Vesicles functionalised with DNA nanostructures featuring different anchoring platforms. Data analysis platform (MATLAB Codes and function)

Version

Software / Usage instructions

Tif images can be opened and analysed in most image visualisation softwares. Channels, in order of appearance as the files are opened, are: Alexa488 (DNA signal), TexasRed (lipid signal), and brightfield. Data analysis codes include a README.txt file with guidance/instructions.

Keywords

DNA nanotechnology, Lipid phase separation, Lipid domains, Partitioning, Artificial cells, Synthetic membranes, Biomimicry

Publisher

Sponsorship
EPSRC (1949809)
Royal Society (UF160152)
Engineering and Physical Sciences Research Council (EP/L015978/1)
Engineering and Physical Sciences Research Council (EP/S022953/1)
R.R.S also acknowledges funding support from the Mexican National Council for Science and Technology (CONACYT, Grant No. 472427) and the Cambridge Trust. L.D.M. also acknowledges funding from the European Research Council (ERC) under the Horizon 2020 Research and Innovation Programme (ERC-STG No 851667 NANOCELL)
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