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dc.contributor.authorZhu, Jinbo
dc.contributor.authorBošković, Filip
dc.contributor.authorNguyen, Bao-Nguyen T
dc.contributor.authorNitschke, Jonathan R
dc.contributor.authorKeyser, Ulrich F
dc.date.accessioned2021-03-27T00:30:36Z
dc.date.available2021-03-27T00:30:36Z
dc.date.issued2021-02-10
dc.identifier.issn1530-6984
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/319273
dc.description.abstractThe toehold-mediated strand displacement reaction (SDR) is a powerful enzyme-free tool for molecular manipulation, DNA computing, signal amplification, etc. However, precise modulation of SDR kinetics without changing the original design remains a significant challenge. We introduce a new means of modulating SDR kinetics using an external stimulus: a water-soluble FeII4L4 tetrahedral cage. Our results show that the presence of a flexible phosphate group and a minimum toehold segment length are essential for FeII4L4 binding to DNA. SDRs mediated by toehold ends in different lengths (3-5) were investigated as a function of cage concentration. Their reaction rates all first increased and then decreased as cage concentration increased. We infer that cage binding on the toehold end slows SDR, whereas the stabilization of intermediates that contain two overhangs accelerates SDR. The tetrahedral cage thus serves as a versatile tool for modulation of SDR kinetics.
dc.description.sponsorshipGeorge and Lilian Schiff Foundation Studentship; Winton Programme for the Physics of Sustainability; St. John’s Benefactors’ Scholarship
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleKinetics of Toehold-Mediated DNA Strand Displacement Depend on FeII4L4 Tetrahedron Concentration.
dc.typeArticle
prism.endingPage1374
prism.issueIdentifier3
prism.publicationDate2021
prism.publicationNameNano Lett
prism.startingPage1368
prism.volume21
dc.identifier.doi10.17863/CAM.66391
dcterms.dateAccepted2020-12-29
rioxxterms.versionofrecord10.1021/acs.nanolett.0c04125
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-02
dc.contributor.orcidZhu, Jinbo [0000-0003-4935-825X]
dc.contributor.orcidBošković, Filip [0000-0001-7663-2408]
dc.contributor.orcidNitschke, Jonathan R [0000-0002-4060-5122]
dc.contributor.orcidKeyser, Ulrich F [0000-0003-3188-5414]
dc.identifier.eissn1530-6992
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/M008258/1)
pubs.funder-project-idEuropean Research Council (647144)
pubs.funder-project-idEuropean Research Council (695009)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/P027067/1)
cam.issuedOnline2021-01-28


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International