Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis.
MetadataShow full item record
Wrobel, L., Siddiqi, F., & Rubinsztein, D. (2021). Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis.. STAR protocols, 2 (2), 100500. https://doi.org/10.1016/j.xpro.2021.100500
We present a protocol for in vivo siRNA-mediated knockdown of a gene-of-interest in mouse liver using systemic delivery by intravenous injection. This protocol describes the detailed methods for delivery of siRNA particles, for feeding/starving cycles and for liver tissue isolation followed by gene expression analysis, measured at the mRNA and protein levels. For complete information on the generation and use of this protocol, please refer to (Wrobel et al., 2020).
We are grateful for funding from the UK Dementia Research Institute (funded by the MRC, Alzheimer’s Research UK and the Alzheimer’s Society) (to DCR), the Roger de Spoelberch Foundation (DCR), the European Molecular Biology Organisation (EMBO long-term fellowship to LW ALTF 135-2016). We thank the University of Cambridge core mice facility staff.
Embargo Lift Date
External DOI: https://doi.org/10.1016/j.xpro.2021.100500
This record's URL: https://www.repository.cam.ac.uk/handle/1810/319559
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/