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dc.contributor.authorOwen, Daviden
dc.contributor.authorBriggs, Johnen
dc.contributor.authorMorado, Dustinen
dc.contributor.authorKovtun, Oleksiyen
dc.contributor.authorLeneva, Natalyaen
dc.date.accessioned2021-04-14T13:20:26Z
dc.date.available2021-04-14T13:20:26Z
dc.date.issued2021-03-24en
dc.identifier.issn2375-2548
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/319867
dc.description.abstractRetromer is a master regulator of cargo retrieval from endosomes, which is critical for many cellular processes including signalling, immunity, neuroprotection and virus infection. The retromer core (VPS26/VPS29/VPS35) is present on cargo-transporting, tubular carriers along with a range of sorting nexins. Here, we elucidate the structural basis of membrane tubulation and coupled cargo recognition by metazoan and fungal retromer coats assembled with the non-BAR sorting nexin SNX3 using cryo-electron tomography. The retromer core retains its arched, scaffolding structure but changes its mode of membrane recruitment when assembled with different SNX adaptors allowing cargo recognition at subunit interfaces. Thus membrane bending and cargo incorporation can be modulated to allow retromer to traffic cargoes along different cellular transport routes.
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleArchitecture and mechanism of metazoan retromer:SNX3 tubular coat assemblyen
dc.typeArticle
prism.publicationDate2021en
prism.publicationNameScience Advancesen
dc.identifier.doi10.17863/CAM.66992
dcterms.dateAccepted2021-01-30en
rioxxterms.versionofrecord10.1126/sciadv.abf8598en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2021-03-24en
dc.contributor.orcidOwen, David [0000-0002-8351-6322]
dc.contributor.orcidLeneva, Natalya [0000-0001-8102-9769]
dc.identifier.eissn2375-2548
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (202905/Z/16/Z)
pubs.funder-project-idWellcome Trust (207455/Z/17/Z)
cam.issuedOnline2021-03-24en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International