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dc.contributor.authorBryant, Owain J
dc.contributor.authorChung, Betty Y-W
dc.contributor.authorFraser, Gillian M
dc.date.accessioned2021-04-22T23:30:40Z
dc.date.available2021-04-22T23:30:40Z
dc.date.issued2021-08
dc.identifier.issn0950-382X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/321480
dc.description.abstractBacterial flagellar subunits are exported across the cell membrane by the flagellar Type III Secretion System (fT3SS), powered by the proton motive force (pmf) and a specialized ATPase that enables the flagellar export gate to utilize the pmf electric potential (ΔΨ). Export gate activation is mediated by the ATPase stalk, FliJ, but how this process is regulated to prevent wasteful dissipation of pmf in the absence of subunit cargo is not known. Here, we show that FliJ activation of the export gate is regulated by flagellar export chaperones. FliJ binds unladen chaperones and, by using novel chaperone variants specifically defective for FliJ binding, we show that disruption of this interaction attenuates motility and cognate subunit export. We demonstrate in vitro that chaperones and the FlhA export gate component compete for binding to FliJ, and show in vivo that unladen chaperones, which would be present in the cell when subunit levels are low, sequester FliJ to prevent activation of the export gate and attenuate subunit export. Our data indicate a mechanism whereby chaperones couple availability of subunit cargo to pmf-driven export by the fT3SS.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherWiley
dc.rightsAll rights reserved
dc.titleChaperone-mediated coupling of subunit availability to activation of flagellar Type III secretion.
dc.typeArticle
prism.endingPage549
prism.issueIdentifier2
prism.publicationDate2021
prism.publicationNameMol Microbiol
prism.startingPage538
prism.volume116
dc.identifier.doi10.17863/CAM.68599
dcterms.dateAccepted2021-04-21
rioxxterms.versionofrecord10.1111/mmi.14731
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-08
dc.contributor.orcidFraser, Gillian M [0000-0002-4874-8734]
dc.identifier.eissn1365-2958
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/M007197/1)
pubs.funder-project-idMedical Research Council (MR/R021821/1)
pubs.funder-project-idIsaac Newton Trust (19.07(h))
cam.issuedOnline2021-06-23
cam.orpheus.successMon May 03 07:30:23 BST 2021 - Embargo updated
cam.orpheus.counter1
rioxxterms.freetoread.startdate2022-04-23


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