Expected values for gastrointestinal and pancreatic hormone concentrations in healthy volunteers in the fasting and postprandial state.
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Publication Date
2021-03Journal Title
Ann Clin Biochem
ISSN
0004-5632
Publisher
SAGE Publications
Volume
58
Issue
2
Pages
108-116
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Meek, C., Lewis, H. B., Burling, K., Reimann, F., & Gribble, F. (2021). Expected values for gastrointestinal and pancreatic hormone concentrations in healthy volunteers in the fasting and postprandial state.. Ann Clin Biochem, 58 (2), 108-116. https://doi.org/10.1177/0004563220975658
Abstract
BACKGROUND: Gastrointestinal hormones regulate intestinal transit, control digestion, influence appetite and promote satiety. Altered production or action of gut hormones, including glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP) and peptide YY (PYY), may contribute to the biological basis of obesity and altered glucose homeostasis. However, challenges in analytical methodology and lack of clarity on expected values for healthy individuals have limited progress in this field. The aim of this study was to describe expected concentrations of gastrointestinal and pancreatic hormones in healthy volunteers following a standardized meal test (SMT) or 75 g oral glucose tolerance test (OGTT). METHODS: A total of 28 healthy volunteers (12 men, 16 women; mean age 31.3 years; mean body mass index 24.9 kg/m2) were recruited to attend a hospital clinic on two occasions. Volunteers had blood sampling in the fasting state and were given, in randomized order, an oral glucose tolerance test (OGTT) and standardized mixed liquid meal test with venepuncture at timed intervals for 4 h after ingestion. Analytical methods for gut and pancreatic hormones were assessed and optimized. Concentrations of gut and pancreatic hormones were measured and used to compile ranges of expected values. RESULTS: Ranges of expected values were created for glucose, insulin, glucagon, GLP-1, GIP, PYY and free fatty acids in response to a standardized mixed liquid meal or OGTT. Intact proinsulin and C-peptide levels were also measured following the OGTT. CONCLUSIONS: These ranges of expected values can now be used to compare gut hormone concentrations between healthy individuals and patient groups.
Keywords
Humans, Obesity, Gastrointestinal Hormones, Gastric Inhibitory Polypeptide, Peptide YY, Pancreatic Hormones, Glucagon, Insulin, Blood Glucose, Glucose Tolerance Test, Fasting, Postprandial Period, Reference Values, Adolescent, Adult, Aged, Middle Aged, Female, Male, Glucagon-Like Peptide 1, Young Adult, Healthy Volunteers
Sponsorship
Medical Research Council (MC_UU_12012/3)
Wellcome Trust (100574/Z/12/Z)
Medical Research Council (MC_UU_12012/5)
Wellcome Trust (106262/Z/14/Z)
MRC (MC_UU_00014/3)
MRC (MC_UU_00014/5)
Identifiers
External DOI: https://doi.org/10.1177/0004563220975658
This record's URL: https://www.repository.cam.ac.uk/handle/1810/321747
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