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dc.contributor.authorCortés, Alba
dc.contributor.authorWills, John
dc.contributor.authorSu, Xiaopei
dc.contributor.authorHewitt, Rachel E.
dc.contributor.authorRobertson, Jack
dc.contributor.authorScotti, Riccardo
dc.contributor.authorPrice, Daniel R. G.
dc.contributor.authorBartley, Yvonne
dc.contributor.authorMcNeilly, Tom N.
dc.contributor.authorKrause, Lutz
dc.contributor.authorPowell, Jonathan J.
dc.contributor.authorNisbet, Alasdair J.
dc.contributor.authorCantacessi, Cinzia
dc.date.accessioned2021-04-30T15:29:25Z
dc.date.available2021-04-30T15:29:25Z
dc.date.issued2020-04-30
dc.date.submitted2019-11-23
dc.identifier.others40168-020-00818-9
dc.identifier.other818
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/321780
dc.description.abstractAbstract: Background: The multifaceted interactions between gastrointestinal (GI) helminth parasites, host gut microbiota and immune system are emerging as a key area of research within the field of host-parasite relationships. In spite of the plethora of data available on the impact that GI helminths exert on the composition of the gut microflora, whether alterations of microbial profiles are caused by direct parasite-bacteria interactions or, indirectly, by alterations of the GI environment (e.g. mucosal immunity) remains to be determined. Furthermore, no data is thus far available on the downstream roles that qualitative and quantitative changes in gut microbial composition play in the overall pathophysiology of parasite infection and disease. Results: In this study, we investigated the fluctuations in microbiota composition and local immune microenvironment of sheep vaccinated against, and experimentally infected with, the ‘brown stomach worm’ Teladorsagia circumcincta, a parasite of worldwide socio-economic significance. We compared the faecal microbial profiles of vaccinated and subsequently infected sheep with those obtained from groups of unvaccinated/infected and unvaccinated/uninfected animals. We show that alterations of gut microbial composition are associated mainly with parasite infection, and that this involves the expansion of populations of bacteria with known pro-inflammatory properties that may contribute to the immunopathology of helminth disease. Using novel quantitative approaches for the analysis of confocal microscopy-derived images, we also show that gastric tissue infiltration of T cells is driven by parasitic infection rather than anti-helminth vaccination. Conclusions: Teladorsagia circumcincta infection leads to an expansion of potentially pro-inflammatory gut microbial species and abomasal T cells. This data paves the way for future experiments aimed to determine the contribution of the gut flora to the pathophysiology of parasitic disease, with the ultimate aim to design and develop novel treatment/control strategies focused on preventing and/or restricting bacterial-mediated inflammation upon infection by GI helminths. DCvTgHLrMa4pPTvhPWGjhoVideo Abstract
dc.languageen
dc.publisherBioMed Central
dc.subjectResearch
dc.subjectGastrointestinal helminth
dc.subjectTeladorsagia circumcincta
dc.subjectParasite gastroenteritis
dc.subjectGut microbiota
dc.subjectBacterial 16S rRNA gene sequencing
dc.subjectVaccine
dc.subjectPathobiont
dc.subjectT cell
dc.subjectAutomated in situ cell counting
dc.titleInfection with the sheep gastrointestinal nematode Teladorsagia circumcincta increases luminal pathobionts
dc.typeArticle
dc.date.updated2021-04-30T15:29:20Z
prism.issueIdentifier1
prism.publicationNameMicrobiome
prism.volume8
dc.identifier.doi10.17863/CAM.69237
dcterms.dateAccepted2020-03-02
rioxxterms.versionofrecord10.1186/s40168-020-00818-9
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2049-2618
pubs.funder-project-idFundación Alfonso Martín Escudero (N/A)
pubs.funder-project-idIsaac Newton Trust (N/A)
pubs.funder-project-idRural and Environment Science and Analytical Services Division (N/A)
pubs.funder-project-idHorizon 2020 Framework Programme (635408)
pubs.funder-project-idMedical Research Council (MR/R005699/1)


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