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dc.contributor.authorLiu, Ye
dc.contributor.authorDabrowska, Catherine
dc.contributor.authorMavousian, Antranik
dc.contributor.authorStrauss, Bernhard
dc.contributor.authorMeng, Fanlong
dc.contributor.authorMazzaglia, Corrado
dc.contributor.authorOuaras, Karim
dc.contributor.authorMacintosh, Callum
dc.contributor.authorTerentjev, Eugene
dc.contributor.authorLee, Joo‐Hyeon
dc.contributor.authorHuang, Yan Yan Shery
dc.date.accessioned2021-05-05T09:16:51Z
dc.date.available2021-05-05T09:16:51Z
dc.date.issued2021-02-08
dc.date.submitted2020-08-31
dc.identifier.issn2198-3844
dc.identifier.otheradvs2391
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/321956
dc.description.abstractAbstract: Epithelial, stem‐cell derived organoids are ideal building blocks for tissue engineering, however, scalable and shape‐controlled bio‐assembly of epithelial organoids into larger and anatomical structures is yet to be achieved. Here, a robust organoid engineering approach, Multi‐Organoid Patterning and Fusion (MOrPF), is presented to assemble individual airway organoids of different sizes into upscaled, scaffold‐free airway tubes with predefined shapes. Multi‐Organoid Aggregates (MOAs) undergo accelerated fusion in a matrix‐depleted, free‐floating environment, possess a continuous lumen, and maintain prescribed shapes without an exogenous scaffold interface. MOAs in the floating culture exhibit a well‐defined three‐stage process of inter‐organoid surface integration, luminal material clearance, and lumina connection. The observed shape stability of patterned MOAs is confirmed by theoretical modelling based on organoid morphology and the physical forces involved in organoid fusion. Immunofluorescent characterization shows that fused MOA tubes possess an unstratified epithelium consisting mainly of tracheal basal stem cells. By generating large, shape‐controllable organ tubes, MOrPF enables upscaled organoid engineering towards integrated organoid devices and structurally complex organ tubes.
dc.languageen
dc.subjectFull Paper
dc.subjectFull Papers
dc.subjectbio‐assembly
dc.subjectorganoids
dc.subjectrespiratory system
dc.subjecttissue engineering
dc.subjecttissue morphogenesis
dc.titleBio‐assembling Macro‐Scale, Lumenized Airway Tubes of Defined Shape via Multi‐Organoid Patterning and Fusion
dc.typeArticle
dc.date.updated2021-05-05T09:16:50Z
prism.issueIdentifier9
prism.publicationNameAdvanced Science
prism.volume8
dc.identifier.doi10.17863/CAM.69413
rioxxterms.versionofrecord10.1002/advs.202003332
rioxxterms.versionAO
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidLee, Joo‐Hyeon [0000-0002-7364-6422]
dc.contributor.orcidHuang, Yan Yan Shery [0000-0003-2619-730X]
pubs.funder-project-idEuropean Research Council (758865, 679411)
pubs.funder-project-idWellcome Trust (107633/Z/15/Z)


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