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Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Pietras, Zbigniew 
Hardwick, Steven W 
Swiezewski, Szymon 
Luisi, Ben F 

Abstract

Members of the DEAD-box family of RNA helicases contribute to virtually every aspect of RNA metabolism, in organisms from all domains of life. Many of these helicases are constituents of multicomponent assemblies, and their interactions with partner proteins within the complexes underpin their activities and biological function. In Escherichia coli the DEAD-box helicase RhlB is a component of the multienzyme RNA degradosome assembly, and its interaction with the core ribonuclease RNase E boosts the ATP-dependent activity of the helicase. Earlier studies have identified the regulator of ribonuclease activity A (RraA) as a potential interaction partner of both RNase E and RhlB. We present structural and biochemical evidence showing how RraA can bind to, and modulate the activity of RhlB and another E. coli DEAD-box enzyme, SrmB. Crystallographic structures are presented of RraA in complex with a portion of the natively unstructured C-terminal tail of RhlB at 2.8-Å resolution, and in complex with the C-terminal RecA-like domain of SrmB at 2.9 Å. The models suggest two distinct mechanisms by which RraA might modulate the activity of these and potentially other helicases.

Description

Keywords

Analytical Ultracentrifugation, Protein-Protein Interactions, RNA Helicase, RNA-binding Protein, Regulator of Ribonuclease Activity A, RhlB, RraA, SrmB, X-ray Crystallography, Crystallography, X-Ray, DEAD-box RNA Helicases, Endoribonucleases, Escherichia coli, Escherichia coli Proteins, Models, Molecular, Protein Binding, Protein Structure, Quaternary, Protein Structure, Tertiary

Journal Title

J Biol Chem

Conference Name

Journal ISSN

0021-9258
1083-351X

Volume Title

288

Publisher

Elsevier BV