Repository logo
 

Research data in support of:"Responsive core-shell DNA particles trigger lipid-membrane disruption and bacteria entrapment"


Change log

Authors

Brady, Ryan 
Mancini, Leonardo 
Contini, Claudia 
Rubio Sanchez, Roger  ORCID logo  https://orcid.org/0000-0001-5574-5809

Description

DLS data / images of agarose gels with various DNA constructs proving their successful folding and showing triggered corona displacement from core-shell particles. UV melting curves of non-cholesterolized DNA motifs. Confocal micrographs of DNA particles with fluorescent core and corona, vesicles ruptured and permeabilized by triggered DNA particles, E. coli entrapped in a DNA net formed by triggered DNA particles. TEM micrographs of core-shell DNA particles. Fast frame rate bright-field videos and bright-field images of non-triggered / triggered DNA particles, E. coli entrapped in a DNA net. Epifluorescence micrographs of of non-triggered / triggered DNA particles, E. coli entrapped in a DNA net. Fluorescence measurements showing the progressive leakage of calcein from LUVs. Absorbance measurements used to calculate the lipid concentration in GUVs and LUVs. Optical density measurements showing the bacterial growth in the presence of core-shell DNA particles.

Version

Software / Usage instructions

TIFF and PNG files can be opened and analysed using most of the available image visualisation / analysis software programs (examples include ImageJ and Matlab). CSV files can be directly opened with Microsoft Excel or imported into any text data analysis software program (for example, Matlab or Jupyter Notebook). TXT files including detailed description of the data can be opened using Notepad.

Keywords

DNA nanotechnology, Core-shell DNA nanoparticles, Synthetic cells, Biomimicry, Lipid membrane rupture, Lipid membrane permeabilization, Bacteria entrapment

Publisher

Sponsorship
EPSRC (2127174)
Royal Society (UF160152)
Relationships
Supplements: