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Placental secretome characterization identifies candidates for pregnancy complications

Published version
Peer-reviewed

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Authors

Napso, Tina 
Lligoña, Marta Ibañez  ORCID logo  https://orcid.org/0000-0003-3428-2168
Kay, Richard G. 

Abstract

Abstract: Alterations in maternal physiological adaptation during pregnancy lead to complications, including abnormal birthweight and gestational diabetes. Maternal adaptations are driven by placental hormones, although the full identity of these is lacking. This study unbiasedly characterized the secretory output of mouse placental endocrine cells and examined whether these data could identify placental hormones important for determining pregnancy outcome in humans. Secretome and cell peptidome analyses were performed on cultured primary trophoblast and fluorescence-activated sorted endocrine trophoblasts from mice and a placental secretome map was generated. Proteins secreted from the placenta were detectable in the circulation of mice and showed a higher relative abundance in pregnancy. Bioinformatic analyses showed that placental secretome proteins are involved in metabolic, immune and growth modulation, are largely expressed by human placenta and several are dysregulated in pregnancy complications. Moreover, proof-of-concept studies found that secreted placental proteins (sFLT1/MIF and ANGPT2/MIF ratios) were increased in women prior to diagnosis of gestational diabetes. Thus, placental secretome analysis could lead to the identification of new placental biomarkers of pregnancy complications.

Description

Keywords

Article, /631/136/3194, /692/163/2743/2730, /631/1647/48, /631/1647/2067, /631/443/494/2732/2730, /13/106, /13/31, /38/71, /38/77, /82/58, article

Journal Title

Communications Biology

Conference Name

Journal ISSN

2399-3642

Volume Title

4

Publisher

Nature Publishing Group UK
Sponsorship
Royal Society (DH130036)
Academy of Medical Sciences (SBF002/1028)
Isaac Newton Trust (RG97390)
Lister Institute of Preventive Medicine (RG93692)