YbeY is required for ribosome small subunit assembly and tRNA processing in human mitochondria.
D'Souza, Aaron R
Van Haute, Lindsey
Powell, Christopher A
Mutti, Christian D
Nucleic acids research
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D'Souza, A. R., Van Haute, L., Powell, C. A., Mutti, C. D., Páleníková, P., Rebelo-Guiomar, P., Rorbach, J., & et al. (2021). YbeY is required for ribosome small subunit assembly and tRNA processing in human mitochondria.. Nucleic acids research, 49 (10), 5798-5812. https://doi.org/10.1093/nar/gkab404
Mitochondria contain their own translation apparatus which enables them to produce the polypeptides encoded in their genome. The mitochondrially-encoded RNA components of the mitochondrial ribosome require various post-transcriptional processing steps. Additional protein factors are required to facilitate the biogenesis of the functional mitoribosome. We have characterized a mitochondrially-localized protein, YbeY, which interacts with the assembling mitoribosome through the small subunit. Loss of YbeY leads to a severe reduction in mitochondrial translation and a loss of cell viability, associated with less accurate mitochondrial tRNASer(AGY) processing from the primary transcript and a defect in the maturation of the mitoribosomal small subunit. Our results suggest that YbeY performs a dual, likely independent, function in mitochondria being involved in precursor RNA processing and mitoribosome biogenesis. Issue Section: Nucleic Acid Enzymes.
Mitochondria, Humans, Ribonucleases, Mitochondrial Proteins, RNA, Transfer, Immunohistochemistry, Sequence Alignment, Cell Survival, Protein Biosynthesis, RNA Processing, Post-Transcriptional, Amino Acid Sequence, Mass Spectrometry, Ribosome Subunits, Small, Gene Knockout Techniques, HEK293 Cells, Mitochondrial Ribosomes
Medical Research Council (MC_UU_00015/4)
External DOI: https://doi.org/10.1093/nar/gkab404
This record's URL: https://www.repository.cam.ac.uk/handle/1810/324469
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/