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dc.contributor.authorDel Rosario, Joanne Marie M.
dc.contributor.authorda Costa, Kelly A. S.
dc.contributor.authorAsbach, Benedikt
dc.contributor.authorFerrara, Francesca
dc.contributor.authorFerrari, Matteo
dc.contributor.authorWells, David A.
dc.contributor.authorMann, Gurdip Singh
dc.contributor.authorAmeh, Veronica O.
dc.contributor.authorSabeta, Claude T.
dc.contributor.authorBanyard, Ashley C.
dc.contributor.authorKinsley, Rebecca
dc.contributor.authorScott, Simon D.
dc.contributor.authorWagner, Ralf
dc.contributor.authorHeeney, Jonathan L.
dc.contributor.authorCarnell, George W.
dc.contributor.authorTemperton, Nigel J.
dc.date.accessioned2021-07-07T14:05:59Z
dc.date.available2021-07-07T14:05:59Z
dc.date.issued2021-07-05
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/324915
dc.description.abstractWe developed an influenza hemagglutinin (HA) pseudotype library encompassing Influenza A subtypes HA1-18 and Influenza B subtypes (both lineages) to be employed in influenza pseudotype microneutralization (pMN) assays. The pMN is highly sensitive and specific for detecting virus-specific neutralizing antibodies against influenza viruses and can be used to assess antibody functionality in vitro. Here we show the production of these viral HA pseudotypes and their employment as substitutes for wildtype viruses in influenza neutralization assays. We demonstrate their utility in detecting serum responses to vaccination with the ability to evaluate cross-subtype neutralizing responses elicited by specific vaccinating antigens. Our findings may inform further preclinical studies involving immunization dosing regimens in mice and may help in the creation and selection of better antigens for vaccine design. These HA pseudotypes can be harnessed to meet strategic objectives that contribute to the strengthening of global influenza surveillance, expansion of seasonal influenza prevention and control policies, and strengthening pandemic preparedness and response.
dc.languageen
dc.publisherMDPI
dc.subjectinfluenza
dc.subjecthemagglutinin
dc.subjectpseudotype
dc.subjectvaccine
dc.subjectimmunogenicity
dc.subjectmonoclonal antibody
dc.subjectneutralization
dc.titleExploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection
dc.typeArticle
dc.date.updated2021-07-07T14:05:58Z
prism.issueIdentifier7
prism.publicationNameVaccines
prism.volume9
dc.identifier.doi10.17863/CAM.72369
dcterms.dateAccepted2021-06-21
rioxxterms.versionofrecord10.3390/vaccines9070741
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidda Costa, Kelly A. S. [0000-0001-6352-3843]
dc.contributor.orcidAsbach, Benedikt [0000-0003-1056-8591]
dc.contributor.orcidFerrara, Francesca [0000-0002-4973-1520]
dc.contributor.orcidBanyard, Ashley C. [0000-0002-1286-9825]
dc.contributor.orcidScott, Simon D. [0000-0002-8290-0461]
dc.contributor.orcidCarnell, George W. [0000-0001-8875-0989]
dc.contributor.orcidTemperton, Nigel J. [0000-0002-7978-3815]
dc.identifier.eissn2076-393X
pubs.funder-project-idInnovate UK (105078)
pubs.funder-project-idBill and Melinda Gates Foundation (G101404)


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