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dc.contributor.authorTidswell, Olivia RA
dc.contributor.authorBenton, Matthew A
dc.contributor.authorAkam, Michael
dc.date.accessioned2021-07-26T23:31:08Z
dc.date.available2021-07-26T23:31:08Z
dc.date.issued2021-08-15
dc.identifier.issn0950-1991
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/325413
dc.description.abstractThe neuroblast timer genes hunchback, Krüppel, nubbin and castor are expressed in temporal sequence in neural stem cells, and in corresponding spatial sequence along the Drosophila blastoderm. As canonical gap genes, hunchback and Krüppel play a crucial role in insect segmentation, but the roles of nubbin and castor in this process remain ambiguous. We have investigated the expression and functions of nubbin and castor during segmentation in the beetle Tribolium. We show that Tc-hunchback, Tc-Krüppel, Tc-nubbin and Tc-castor are expressed sequentially in the segment addition zone, and that Tc-nubbin regulates segment identity redundantly with two previously described gap/gap-like genes, Tc-giant and Tc-knirps. Simultaneous knockdown of Tc-nubbin, Tc-giant and Tc-knirps results in the formation of ectopic legs on abdominal segments. This homeotic transformation is caused by loss of abdominal Hox gene expression, likely due to expanded Tc-Krüppel expression. Our findings support the theory that the neuroblast timer series was co-opted for use in insect segment patterning, and contribute to our growing understanding of the evolution and function of the gap gene network outside of Drosophila.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherThe Company of Biologists
dc.rightsAll rights reserved
dc.titleThe neuroblast timer gene nubbin exhibits functional redundancy with gap genes to regulate segment identity in Tribolium.
dc.typeArticle
prism.issueIdentifier16
prism.publicationDate2021
prism.publicationNameDevelopment
prism.volume148
dc.identifier.doi10.17863/CAM.72869
dcterms.dateAccepted2021-07-23
rioxxterms.versionofrecord10.1242/dev.199719
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-08-20
dc.contributor.orcidTidswell, Olivia RA [0000-0003-2068-9856]
dc.contributor.orcidBenton, Matthew A [0000-0001-7953-0765]
dc.contributor.orcidAkam, Michael [0000-0003-0063-2297]
dc.identifier.eissn1477-9129
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (109147/Z/15/Z)
cam.issuedOnline2021-08-20
cam.orpheus.successMon Aug 23 07:30:43 BST 2021 - Embargo updated
cam.orpheus.counter2
rioxxterms.freetoread.startdate2022-08-20


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