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dc.contributor.authorIslam, Md Saiful
dc.contributor.authorBandyra, Katarzyna J
dc.contributor.authorChao, Yanjie
dc.contributor.authorVogel, Jörg
dc.contributor.authorLuisi, Ben
dc.date.accessioned2021-07-27T23:30:12Z
dc.date.available2021-07-27T23:30:12Z
dc.date.issued2021-11
dc.identifier.issn1355-8382
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/325421
dc.description.abstractThe conserved endoribonuclease RNase E dominates the dynamic landscape of RNA metabolism and underpins control mediated by small regulatory RNAs in diverse bacterial species. We explored the enzyme's hydrolytic mechanism, allosteric activation, and interplay with partner proteins in the multicomponent RNA degradosome assembly of Escherichia coli. RNase E cleaves single-stranded RNA with preference to attack the phosphate located at the 5' nucleotide preceding uracil, and we corroborate key interactions that select that base. Unexpectedly, RNase E activity is impeded strongly when the recognized uracil is isomerized to 5-ribosyluracil (pseudouridine), from which we infer the detailed geometry of the hydrolytic attack process. Kinetics analyses support models for recognition of secondary structure in substrates by RNase E and for allosteric autoregulation. The catalytic power of the enzyme is boosted when it is assembled into the multienzyme RNA degradosome, most likely as a consequence of substrate capture and presentation. Our results rationalize the origins of substrate preferences of RNase E and illuminate its catalytic mechanism, supporting the roles of allosteric domain closure and cooperation with other components of the RNA degradosome complex.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherCold Spring Harbor Laboratory
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleImpact of pseudouridylation, substrate fold, and degradosome organization on the endonuclease activity of RNase E.
dc.typeArticle
prism.endingPage1352
prism.issueIdentifier11
prism.publicationDate2021
prism.publicationNameRNA
prism.startingPage1339
prism.volume27
dc.identifier.doi10.17863/CAM.72878
dcterms.dateAccepted2021-07-26
rioxxterms.versionofrecord10.1261/rna.078840.121
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-11
dc.contributor.orcidChao, Yanjie [0000-0002-5735-7954]
dc.contributor.orcidLuisi, Ben [0000-0003-1144-9877]
dc.identifier.eissn1469-9001
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (200873/Z/16/Z)
cam.issuedOnline2021-08-02
cam.orpheus.successMon Aug 16 07:30:54 BST 2021 - Embargo updated
cam.orpheus.counter1
rioxxterms.freetoread.startdate2021-08-02


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CC BY 4.0
Except where otherwise noted, this item's licence is described as CC BY 4.0