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dc.contributor.authorSchoonejans, Josca
dc.contributor.authorBlackmore, Heather L
dc.contributor.authorAshmore, Thomas J
dc.contributor.authorAiken, Catherine
dc.contributor.authorTwinn, Denise
dc.contributor.authorOzanne, Susan
dc.date.accessioned2021-07-27T23:30:13Z
dc.date.available2021-07-27T23:30:13Z
dc.date.issued2021-07-28
dc.identifier.issn1661-6596
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/325422
dc.description.abstractBACKGROUND: Metformin is commonly used to treat gestational diabetes mellitus. This study investigated the effect of maternal metformin intervention during obese glucose-intolerant pregnancy on the gonadal white adipose tissue (WAT) of 8-week-old male and female mouse offspring. METHODS: C57BL/6J female mice were provided with a control (Con) or obesogenic diet (Ob) to induce pre-conception obesity. Half the obese dams were treated orally with 300 mg/kg/d of metformin (Ob-Met) during pregnancy. Gonadal WAT depots from 8-week-old offspring were investigated for adipocyte size, macrophage infiltration and mRNA expression of pro-inflammatory genes using RT-PCR. RESULTS: Gestational metformin attenuated the adiposity in obese dams and increased the gestation length without correcting the offspring in utero growth restriction and catch-up growth caused by maternal obesity. Despite similar body weight, the Ob and Ob-Met offspring of both sexes showed adipocyte hypertrophy in young adulthood. Male Ob-Met offspring had increased WAT depot weight (p < 0.05), exaggerated adipocyte hyperplasia (p < 0.05 vs. Con and Ob offspring), increased macrophage infiltration measured via histology (p < 0.05) and the mRNA expression of F4/80 (p < 0.05). These changes were not observed in female Ob-Met offspring. CONCLUSIONS: Maternal metformin intervention during obese pregnancy causes excessive adiposity, adipocyte hyperplasia and WAT inflammation in male offspring, highlighting sex-specific effects of prenatal metformin exposure on offspring WAT.
dc.format.mediumElectronic
dc.languageeng
dc.publisherMDPI AG
dc.rightsAll rights reserved
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectAnimals, Newborn
dc.subjectMice
dc.subjectDiabetes, Gestational
dc.subjectPrenatal Exposure Delayed Effects
dc.subjectMetformin
dc.subjectSex Factors
dc.subjectPregnancy
dc.subjectFemale
dc.subjectMale
dc.subjectAdiposity
dc.subjectObesity, Maternal
dc.titleMaternal Metformin Intervention during Obese Glucose-Intolerant Pregnancy Affects Adiposity in Young Adult Mouse Offspring in a Sex-Specific Manner.
dc.typeArticle
prism.issueIdentifier15
prism.publicationDate2021
prism.publicationNameInt J Mol Sci
prism.volume22
dc.identifier.doi10.17863/CAM.72879
dcterms.dateAccepted2021-07-26
rioxxterms.versionofrecord10.3390/ijms22158104
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-07-28
dc.contributor.orcidSchoonejans, Josca [0000-0003-2893-7199]
dc.contributor.orcidBlackmore, Heather L [0000-0003-0180-3852]
dc.contributor.orcidAiken, Catherine [0000-0002-6510-5626]
dc.contributor.orcidTwinn, Denise [0000-0003-2610-277X]
dc.contributor.orcidOzanne, Susan [0000-0001-8753-5144]
dc.identifier.eissn1422-0067
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBritish Heart Foundation (RG/17/12/33167)
pubs.funder-project-idBritish Heart Foundation (None)
pubs.funder-project-idMRC (MC_UU_00014/4)
pubs.funder-project-idMRC (MR/T016701/1)
pubs.funder-project-idMedical Research Council (MC_UU_12012/4)
cam.issuedOnline2021-07-28
cam.orpheus.counter1
rioxxterms.freetoread.startdate2024-07-27


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