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Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7.

Published version
Peer-reviewed

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Type

Article

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Authors

Meng, Bo 
Kemp, Steven A 
Papa, Guido 
Datir, Rawlings 
Ferreira, Isabella A T M 

Abstract

We report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of receptor binding motif replacements such as N439K and Y453F, known to increase binding affinity to the ACE2 receptor and confer antibody escape. In vitro, we show that, although ΔH69/V70 itself is not an antibody evasion mechanism, it increases infectivity associated with enhanced incorporation of cleaved spike into virions. ΔH69/V70 is able to partially rescue infectivity of spike proteins that have acquired N439K and Y453F escape mutations by increased spike incorporation. In addition, replacement of the H69 and V70 residues in the Alpha variant B.1.1.7 spike (where ΔH69/V70 occurs naturally) impairs spike incorporation and entry efficiency of the B.1.1.7 spike pseudotyped virus. Alpha variant B.1.1.7 spike mediates faster kinetics of cell-cell fusion than wild-type Wuhan-1 D614G, dependent on ΔH69/V70. Therefore, as ΔH69/V70 compensates for immune escape mutations that impair infectivity, continued surveillance for deletions with functional effects is warranted.

Description

Funder: Wellcome Trust

Keywords

Resistance, Deletion, Infectivity, Neutralizing Antibodies, Antibody Escape, Covid-19, Sars-cov-2, B.1.1.7, Spike Mutation, Alpha Variant, Cell Line, Vero Cells, Animals, Humans, Recurrence, Antibodies, Viral, Phylogeny, Protein Binding, Mutation, Immune Evasion, Antibodies, Neutralizing, HEK293 Cells, Pandemics, Spike Glycoprotein, Coronavirus, Chlorocebus aethiops, COVID-19, SARS-CoV-2

Journal Title

Cell reports

Conference Name

Journal ISSN

2211-1247

Volume Title

35

Publisher