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Localization of Organelle Proteins by Isotope Tagging: Current status and potential applications in drug discovery research.

Accepted version
Peer-reviewed

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Type

Article

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Authors

Elzek, Mohamed AW 
Christopher, Josie A 
Breckels, Lisa M 
Lilley, Kathryn S 

Abstract

Spatial proteomics has provided important insights into the relationship between protein function and subcellular location. Localization of Organelle Proteins by Isotope Tagging (LOPIT) and its variants are proteome-wide techniques, not matched in scale by microscopy-based or proximity tagging-based techniques, allowing holistic mapping of protein subcellular location and re-localization events downstream of cellular perturbations. LOPIT can be a powerful and versatile tool in drug discovery for unlocking important information on disease pathophysiology, drug mechanism of action, and off-target toxicity screenings. Here, we discuss technical concepts of LOPIT with its potential applications in drug discovery and development research.

Description

Keywords

Drug Discovery, Isotopes, Organelles, Proteome, Proteomics

Journal Title

Drug Discov Today Technol

Conference Name

Journal ISSN

1740-6749
1740-6749

Volume Title

Publisher

Elsevier BV

Rights

All rights reserved
Sponsorship
Wellcome Trust (108467/Z/15/Z)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/R505304/1)
Wellcome Trust (110071/Z/15/Z)
European Commission Horizon 2020 (H2020) Research Infrastructures (RI) (823839)