Localization of Organelle Proteins by Isotope Tagging: Current status and potential applications in drug discovery research.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Elzek, Mohamed AW
Christopher, Josie A
Breckels, Lisa M
Lilley, Kathryn S
Abstract
Spatial proteomics has provided important insights into the relationship between protein function and subcellular location. Localization of Organelle Proteins by Isotope Tagging (LOPIT) and its variants are proteome-wide techniques, not matched in scale by microscopy-based or proximity tagging-based techniques, allowing holistic mapping of protein subcellular location and re-localization events downstream of cellular perturbations. LOPIT can be a powerful and versatile tool in drug discovery for unlocking important information on disease pathophysiology, drug mechanism of action, and off-target toxicity screenings. Here, we discuss technical concepts of LOPIT with its potential applications in drug discovery and development research.
Description
Keywords
Drug Discovery, Isotopes, Organelles, Proteome, Proteomics
Journal Title
Drug Discov Today Technol
Conference Name
Journal ISSN
1740-6749
1740-6749
1740-6749
Volume Title
Publisher
Elsevier BV
Publisher DOI
Rights
All rights reserved
Sponsorship
Wellcome Trust (108467/Z/15/Z)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/R505304/1)
Wellcome Trust (110071/Z/15/Z)
European Commission Horizon 2020 (H2020) Research Infrastructures (RI) (823839)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/R505304/1)
Wellcome Trust (110071/Z/15/Z)
European Commission Horizon 2020 (H2020) Research Infrastructures (RI) (823839)