Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome.
Published version
Peer-reviewed
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Repository DOI
Type
Article
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Authors
Leung, Adelaine Kwun-Wai https://orcid.org/0000-0002-2849-4474
Kondo, Yasushi https://orcid.org/0000-0003-2131-575X
Krummel, Daniel A Pomeranz
Li, Jade
Price, Stephen R
Abstract
Cryo-electron microscopy has greatly advanced our understanding of how the spliceosome cycles through different conformational states to conduct the chemical reactions that remove introns from pre-mRNA transcripts. The Cryo-EM structures were built upon decades of crystallographic studies of various spliceosomal RNA-protein complexes. In this review we give an overview of the crystal structures solved in the Nagai group, utilizing many of the strategies to design crystal packing as described in the accompanying paper.
Description
Keywords
RNA-protein complexes, crystallization, spliceosome
Journal Title
Crystals (Basel)
Conference Name
Journal ISSN
2073-4352
2073-4352
2073-4352
Volume Title
11
Publisher
MDPI AG