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Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.

Published version
Peer-reviewed

Type

Book chapter

Change log

Authors

Buckley, Clare E 

Abstract

This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an "anchor" protein and a "bait" protein. Proteins can therefore be directed to specific subcellular compartments, altering biological processes such as cell polarity and signaling. I detail methods for achieving transient expression of fusion proteins encoding the phytochrome heterodimerization system in early zebrafish embryos (Buckley et al., Dev Cell 36(1):117-126, 2016) and describe the imaging parameters used to achieve subcellular light patterning.

Description

Title

Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.

Keywords

Heterodimerization, Optogenetics, Phytochrome, Polarity, Signaling, Zebrafish, Animals, Embryonic Development, Gene Expression, Microinjections, Optogenetics, Plasmids, Protein Transport, RNA, Messenger, Signal Transduction, Transgenes, Vertebrates, Zebrafish

Is Part Of

Methods in Molecular Biology

Book type

Publisher

Springer New York

ISBN

978-1-4939-9008-5
Sponsorship
Wellcome Trust (208758/Z/17/Z)