Microimplantation of foreign materials for assessment of foreign body immune responses and granuloma formation in zebrafish larvae.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Takaki, Kevin K
Abstract
This protocol describes the microimplantation of foreign materials such as schistosome eggs, polymer beads, and other microscopic objects into the small and optically transparent larval zebrafish for the assessment of immune responses, including granuloma formation. This protocol has wide applicability for both fundamental studies on host responses to parasite eggs and other foreign bodies, as well as the testing of potential biomaterials and devices used for human medical implants. For complete details on the use and execution of this protocol, please refer to Takaki et al. (2021a) and (2021b).
Description
Keywords
Material sciences, Microbiology, Microscopy, Model Organisms, Animals, Foreign Bodies, Granuloma, Immunity, Larva, Zebrafish
Journal Title
STAR Protoc
Conference Name
Journal ISSN
2666-1667
2666-1667
2666-1667
Volume Title
2
Publisher
Elsevier BV
Publisher DOI
Sponsorship
Wellcome Trust (103950/Z/14/Z)
National Institutes of Health (NIH) (7R37A1054503-13)
National Institutes of Health (NIH) (7R37A1054503-13)