AMPA receptor anchoring at CA1 synapses is determined by N-terminal domain and TARP γ8 interactions.
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Watson, J. F., Pinggera, A., Ho, H., & Greger, I. H. (2021). AMPA receptor anchoring at CA1 synapses is determined by N-terminal domain and TARP γ8 interactions.. Nature communications, 12 (1) https://doi.org/10.1038/s41467-021-25281-4
AMPA receptor (AMPAR) abundance and positioning at excitatory synapses regulates the strength of transmission. Changes in AMPAR localisation can enact synaptic plasticity, allowing long-term information storage, and is therefore tightly controlled. Multiple mechanisms regulating AMPAR synaptic anchoring have been described, but with limited coherence or comparison between reports, our understanding of this process is unclear. Here, combining synaptic recordings from mouse hippocampal slices and super-resolution imaging in dissociated cultures, we compare the contributions of three AMPAR interaction domains controlling transmission at hippocampal CA1 synapses. We show that the AMPAR C-termini play only a modulatory role, whereas the extracellular N-terminal domain (NTD) and PDZ interactions of the auxiliary subunit TARP γ8 are both crucial, and each is sufficient to maintain transmission. Our data support a model in which γ8 accumulates AMPARs at the postsynaptic density, where the NTD further tunes their positioning. This interplay between cytosolic (TARP γ8) and synaptic cleft (NTD) interactions provides versatility to regulate synaptic transmission and plasticity.
Neurons, Synapses, Animals, Mice, Inbred C57BL, Receptors, AMPA, Nuclear Proteins, Fluorescent Dyes, Imaging, Three-Dimensional, Synaptic Transmission, Amino Acid Sequence, Protein Binding, Neuronal Plasticity, Mutation, Models, Neurological, Protein Multimerization, CA1 Region, Hippocampal, Protein Domains
Biotechnology and Biological Sciences Research Council (BB/N002113/1)
Medical Research Council (MC_U105174197)
External DOI: https://doi.org/10.1038/s41467-021-25281-4
This record's URL: https://www.repository.cam.ac.uk/handle/1810/328520
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/