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dc.contributor.authorTokgoz, Aziz
dc.contributor.authorWang, Shuo
dc.contributor.authorSastry, Priya
dc.contributor.authorSun, Chang
dc.contributor.authorFigg, Nichola
dc.contributor.authorHuang, Yuan
dc.contributor.authorBennett, Martin
dc.contributor.authorSinha, Sanjay
dc.contributor.authorGillard, Jonathan
dc.contributor.authorSutcliffe, Michael
dc.contributor.authorTeng, Zhongzhao
dc.date.accessioned2021-09-27T23:31:08Z
dc.date.available2021-09-27T23:31:08Z
dc.identifier.issn0148-0731
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/328593
dc.description.abstractObjective: Fibre structures and pathological features, e.g., inflammation and glycosaminoglycan (GAG) deposition, are the primary determinants of aortic mechanical properties which are associated with the development of aneurysm. This study is designed to quantify the association of tissue ultimate strength and extensibility with the structural percentage of different components, in particular, GAG, and local fibre orientation. Materials and Methods: Thoracic aortic aneurysm (TAA) tissues from 8 patients were collected. Ninety-six tissue strips of thickened intima, media and adventitia were prepared for uni-extension tests and histopathological examination. Area ratios of collagen, elastin, macrophage and GAG and collagen fibre dispersion were quantified. Results: Collagen, elastin and GAG were layer-dependent and the inflammatory burden in all layers was low. The local GAG ratio was negatively associated with the collagen ratio (r2=0.173, p<0.05), but positively with elastin (r2=0.037, p<0.05). Higher GAG deposition resulted in larger local collagen fibre dispersion in the media and adventitia, but not in the intima. The ultimate stretch in both axial and circumferential directions was exclusively associated with elastin ratio (Axial: r2=0.186, p=0.04; Circumferential: r2=0.175, p=0.04). Multivariate analysis showed that collagen and GAG contents were both associated with ultimate strength in the circumferential direction, but not with the axial direction (Collagen: slope=27.3, GAG: slope=-18.4, r2=0.438, p=0.002). Conclusions: GAG may play important roles in TAA material strength. Their deposition was found to be associated positively with the local collagen fibre dispersion and negatively with ultimate strength in the circumferential direction.
dc.description.sponsorshipEPSRC (EP/P021654/1), NSERC (6799-427538-2012), NIHR Cambridge Biomedical Research Centre (BRC-1215-20014), EPSRC Doctoral Training Award at the University of Cambridge, China Scholarship Council.
dc.publisherAmerican Society of Mechanical Engineers
dc.rightsAll rights reserved
dc.titleAssociation of collagen, elastin, glycosaminoglycans and macrophages with tissue ultimate material strength and stretch in human thoracic aortic aneurysms: a uniaxial tension study
dc.typeArticle
prism.publicationNameJournal of Biomechanical Engineering
dc.identifier.doi10.17863/CAM.76042
dcterms.dateAccepted2021-09-07
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-09-07
dc.contributor.orcidSutcliffe, Michael [0000-0001-9729-4460]
dc.contributor.orcidTeng, Zhongzhao [0000-0003-3973-6157]
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (146281)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/P021654/1)
cam.orpheus.counter8
rioxxterms.freetoread.startdate2024-09-27


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