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dc.contributor.authorRomanos, Michèle
dc.contributor.authorAllio, Guillaume
dc.contributor.authorRoussigné, Myriam
dc.contributor.authorCombres, Léa
dc.contributor.authorEscalas, Nathalie
dc.contributor.authorSoula, Cathy
dc.contributor.authorMédevielle, François
dc.contributor.authorSteventon, Benjamin
dc.contributor.authorTrescases, Ariane
dc.contributor.authorBénazéraf, Bertrand
dc.date.accessioned2021-10-06T01:06:18Z
dc.date.available2021-10-06T01:06:18Z
dc.date.issued2021-10-05
dc.date.submitted2021-01-15
dc.identifier.other66588
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329031
dc.description.abstractAlthough cell-to-cell heterogeneity in gene and protein expression within cell populations has been widely documented, we know little about its biological functions. By studying progenitors of the posterior region of bird embryos, we found that expression levels of transcription factors Sox2 and Bra, respectively involved in neural tube (NT) and mesoderm specification, display a high degree of cell-to-cell heterogeneity. By combining forced expression and downregulation approaches with time-lapse imaging, we demonstrate that Sox2-to-Bra ratio guides progenitor’s motility and their ability to stay in or exit the progenitor zone to integrate neural or mesodermal tissues. Indeed, high Bra levels confer high motility that pushes cells to join the paraxial mesoderm, while high levels of Sox2 tend to inhibit cell movement forcing cells to integrate the NT. Mathematical modeling captures the importance of cell motility regulation in this process and further suggests that randomness in Sox2/Bra cell-to-cell distribution favors cell rearrangements and tissue shape conservation.
dc.languageen
dc.publishereLife Sciences Publications, Ltd
dc.subjectResearch Article
dc.subjectDevelopmental Biology
dc.subjectneuromesodermal progenitors
dc.subjectmorphogenesis
dc.subjectcell motility
dc.subjectheterogeneity
dc.subjectmathematical modeling
dc.subjectbird embryos
dc.subjectOther
dc.titleCell-to-cell heterogeneity in Sox2 and Bra expression guides progenitor motility and destiny
dc.typeArticle
dc.date.updated2021-10-06T01:06:16Z
prism.publicationNameeLife
prism.volume10
dc.identifier.doi10.17863/CAM.76475
dcterms.dateAccepted2021-09-13
rioxxterms.versionofrecord10.7554/elife.66588
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Bronner, Marianne E
datacite.contributor.supervisorsenior_editor: Bronner, Marianne E
dc.contributor.orcidRoussigné, Myriam [0000-0002-4240-4105]
dc.contributor.orcidSteventon, Benjamin [0000-0001-7838-839X]
dc.contributor.orcidBénazéraf, Bertrand [0000-0002-1937-637X]
dc.identifier.eissn2050-084X
pubs.funder-project-idAgence Nationale de la Recherche (MULTIEL ANRJC)
pubs.funder-project-idFondation ARC pour la Recherche sur le Cancer (PJA 20191209486)


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