Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology
Authors
Guna, Alina
Inglis, Alison J.
Putker, Marrit
Bartolami, Eline
Matile, Stefan
Lequeux, Nicolas
Voorhees, Rebecca M.
Edgar, Rachel S.
Publication Date
2021-10-15Journal Title
Nature Communications
Publisher
Nature Publishing Group UK
Volume
12
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Stangherlin, A., Watson, J. L., Wong, D. C. S., Barbiero, S., Zeng, A., Seinkmane, E., Chew, S. P., et al. (2021). Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology. Nature Communications, 12 (1) https://doi.org/10.1038/s41467-021-25942-4
Abstract
Abstract: Between 6–20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For cell viability, and to maintain volume within narrow limits, the daily variation in osmotic potential exerted by changes in the soluble proteome must be counterbalanced. The mechanisms and consequences of this osmotic compensation have not been investigated before. In cultured cells and in tissue we find that compensation involves electroneutral active transport of Na+, K+, and Cl− through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes confer daily variation in electrical activity. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.
Keywords
Article, /631/45/612/1248, /631/80/105, /631/443/592, /96/106, /14, /14/19, /82/58, /64/60, /9/30, article
Sponsorship
RCUK | Medical Research Council (MRC) (MC_UP_1201/4)
Identifiers
s41467-021-25942-4, 25942
External DOI: https://doi.org/10.1038/s41467-021-25942-4
This record's URL: https://www.repository.cam.ac.uk/handle/1810/329505
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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