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dc.contributor.authorStrittmatter, Nicole
dc.contributor.authorKanvatirth, Panchali
dc.contributor.authorInglese, Paolo
dc.contributor.authorRace, Alan M
dc.contributor.authorNilsson, Anna
dc.contributor.authorDannhorn, Andreas
dc.contributor.authorKudo, Hiromi
dc.contributor.authorGoldin, Robert D
dc.contributor.authorLing, Stephanie
dc.contributor.authorWong, Edmond
dc.contributor.authorSeeliger, Frank
dc.contributor.authorSerra, Maria Paola
dc.contributor.authorHoffmann, Scott
dc.contributor.authorMaglennon, Gareth
dc.contributor.authorHamm, Gregory
dc.contributor.authorAtkinson, James
dc.contributor.authorJones, Stewart
dc.contributor.authorBunch, Josephine
dc.contributor.authorAndrén, Per E
dc.contributor.authorTakats, Zoltan
dc.contributor.authorGoodwin, Richard JA
dc.contributor.authorMastroeni, Pietro
dc.date.accessioned2021-10-20T23:30:14Z
dc.date.available2021-10-20T23:30:14Z
dc.date.issued2021-12-01
dc.identifier.issn1044-0305
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329683
dc.description.abstractA more complete and holistic view on host-microbe interactions is needed to understand the physiological and cellular barriers that affect the efficacy of drug treatments and allow the discovery and development of new therapeutics. Here, we developed a multimodal imaging approach combining histopathology with mass spectrometry imaging (MSI) and same section imaging mass cytometry (IMC) to study the effects of Salmonella Typhimurium infection in the liver of a mouse model using the S. Typhimurium strains SL3261 and SL1344. This approach enables correlation of tissue morphology and specific cell phenotypes with molecular images of tissue metabolism. IMC revealed a marked increase in immune cell markers and localization in immune aggregates in infected tissues. A correlative computational method (network analysis) was deployed to find metabolic features associated with infection and revealed metabolic clusters of acetyl carnitines, as well as phosphatidylcholine and phosphatidylethanolamine plasmalogen species, which could be associated with pro-inflammatory immune cell types. By developing an IMC marker for the detection of Salmonella LPS, we were further able to identify and characterize those cell types which contained S. Typhimurium.
dc.description.sponsorshipSwedish Research Council,, the Swedish Foundation for Strategic Research and the Science for Life Laboratory
dc.languageeng
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAll rights reserved
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.subjectSalmonella infection
dc.subjectimaging mass cytometry
dc.subjectmass spectrometry imaging
dc.subjectmultimodal data integration
dc.subjectnetwork analysis
dc.titleHolistic Characterization of a Salmonella Typhimurium Infection Model Using Integrated Molecular Imaging.
dc.typeArticle
prism.publicationDate2021
prism.publicationNameJ Am Soc Mass Spectrom
dc.identifier.doi10.17863/CAM.77129
dcterms.dateAccepted2021-10-15
rioxxterms.versionofrecord10.1021/jasms.1c00240
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-11-12
dc.contributor.orcidStrittmatter, Nicole [0000-0003-1277-9608]
dc.contributor.orcidInglese, Paolo [0000-0001-6179-9643]
dc.contributor.orcidRace, Alan M [0000-0001-8996-2641]
dc.contributor.orcidDannhorn, Andreas [0000-0002-1087-4057]
dc.contributor.orcidLing, Stephanie [0000-0002-1237-091X]
dc.contributor.orcidAndrén, Per E [0000-0002-4062-7743]
dc.contributor.orcidTakats, Zoltan [0000-0002-0795-3467]
dc.contributor.orcidMastroeni, Pietro [0000-0003-3838-4962]
dc.identifier.eissn1879-1123
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/M000982/1)
cam.issuedOnline2021-11-12
cam.orpheus.successMon Nov 22 07:30:40 GMT 2021 - Embargo updated
cam.orpheus.counter4
rioxxterms.freetoread.startdate2022-11-12


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