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dc.contributor.authorDietz, Sabrina
dc.contributor.authorAlmeida, Miguel Vasconcelos
dc.contributor.authorNischwitz, Emily
dc.contributor.authorSchreier, Jan
dc.contributor.authorViceconte, Nikenza
dc.contributor.authorFradera-Sola, Albert
dc.contributor.authorRenz, Christian
dc.contributor.authorCeron-Noriega, Alejandro
dc.contributor.authorUlrich, Helle D
dc.contributor.authorKappei, Dennis
dc.contributor.authorKetting, René F
dc.contributor.authorButter, Falk
dc.date.accessioned2021-10-25T15:11:49Z
dc.date.available2021-10-25T15:11:49Z
dc.date.issued2021-05-11
dc.date.submitted2020-09-10
dc.identifier.issn2041-1723
dc.identifier.others41467-021-22861-2
dc.identifier.other22861
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329852
dc.description.abstractTelomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approach to identify TEBP-1 and TEBP-2, two paralogs expressed in the germline and embryogenesis that associate to telomeres in vitro and in vivo. tebp-1 and tebp-2 mutants display strikingly distinct phenotypes: tebp-1 mutants have longer telomeres than wild-type animals, while tebp-2 mutants display shorter telomeres and a Mortal Germline. Notably, tebp-1;tebp-2 double mutant animals have synthetic sterility, with germlines showing signs of severe mitotic and meiotic arrest. Furthermore, we show that POT-1 forms a telomeric complex with TEBP-1 and TEBP-2, which bridges TEBP-1/-2 with POT-2/MRT-1. These results provide insights into the composition and organization of a telomeric protein complex in C. elegans.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectArticle
dc.subject/631/45/475
dc.subject/631/80/103/560
dc.subject/82/80
dc.subject/82/83
dc.subject/82/111
dc.subject/82/58
dc.subject/14/19
dc.subject/14/32
dc.subject/14/63
dc.subject/38/23
dc.subject/38/35
dc.subject/38/70
dc.subject/64/11
dc.subjectarticle
dc.titleThe double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1.
dc.typeArticle
dc.date.updated2021-10-25T15:11:47Z
prism.issueIdentifier1
prism.publicationNameNat Commun
prism.volume12
dc.identifier.doi10.17863/CAM.77297
dcterms.dateAccepted2021-03-30
rioxxterms.versionofrecord10.1038/s41467-021-22861-2
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidDietz, Sabrina [0000-0003-3227-9264]
dc.contributor.orcidAlmeida, Miguel Vasconcelos [0000-0002-4816-3111]
dc.contributor.orcidViceconte, Nikenza [0000-0002-5101-4125]
dc.contributor.orcidFradera-Sola, Albert [0000-0002-4780-9312]
dc.contributor.orcidKappei, Dennis [0000-0002-3582-2253]
dc.contributor.orcidKetting, René F [0000-0001-6161-5621]
dc.contributor.orcidButter, Falk [0000-0002-7197-7279]
dc.identifier.eissn2041-1723
pubs.funder-project-idDeutsche Forschungsgemeinschaft (German Research Foundation) (407023052/GRK2526/1, 393547839 – SFB 1361)
cam.issuedOnline2021-05-11


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