An <i>in vitro</i> model for the cultivation of polymicrobial biofilms under continuous-flow conditions.
MetadataShow full item record
O'Brien, T. J., Hassan, M. M., Harrison, F., & Welch, M. (2021). An <i>in vitro</i> model for the cultivation of polymicrobial biofilms under continuous-flow conditions.. F1000Research, 10 https://doi.org/10.12688/f1000research.55140.1
The airways of people with cystic fibrosis (CF) are often chronically colonised with a diverse array of bacterial and fungal species. However, little is known about the relative partitioning of species between the planktonic and biofilm modes of growth in the airways. Existing <i>in vivo</i> and <i>in vitro</i> models of CF airway infection are ill-suited for the long-term recapitulation of mixed microbial communities. Here we describe a simple, <i>in vitro</i> continuous-flow model for the cultivation of polymicrobial biofilms and planktonic cultures on different substrata. Our data provide evidence for inter-species antagonism and synergism in biofilm ecology. We further show that the type of substratum on which the biofilms grow has a profound influence on their species composition. This happens without any major alteration in the composition of the surrounding steady-state planktonic community. Our experimentally-tractable model enables the systematic study of planktonic and biofilm communities under conditions that are nutritionally reminiscent of the CF airway microenvironment, something not possible using any existing <i>in vivo</i> models of CF airway infection.
biofilms, Cystic Fibrosis (Cf), Polymicrobial, 3Rs, In Vitro Models
External DOI: https://doi.org/10.12688/f1000research.55140.1
This record's URL: https://www.repository.cam.ac.uk/handle/1810/329894
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: email@example.com