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dc.contributor.authorO'Brien, Thomas James
dc.contributor.authorHassan, Marwa Mohsen
dc.contributor.authorHarrison, Freya
dc.contributor.authorWelch, Martin
dc.date.accessioned2021-10-26T00:46:21Z
dc.date.available2021-10-26T00:46:21Z
dc.date.issued2021-08-13
dc.identifier.issn2046-1402
dc.identifier.otherPMC8442117
dc.identifier.other34557293
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329894
dc.description.abstractThe airways of people with cystic fibrosis (CF) are often chronically colonised with a diverse array of bacterial and fungal species. However, little is known about the relative partitioning of species between the planktonic and biofilm modes of growth in the airways. Existing <i>in vivo</i> and <i>in vitro</i> models of CF airway infection are ill-suited for the long-term recapitulation of mixed microbial communities. Here we describe a simple, <i>in vitro</i> continuous-flow model for the cultivation of polymicrobial biofilms and planktonic cultures on different substrata. Our data provide evidence for inter-species antagonism and synergism in biofilm ecology. We further show that the type of substratum on which the biofilms grow has a profound influence on their species composition. This happens without any major alteration in the composition of the surrounding steady-state planktonic community. Our experimentally-tractable model enables the systematic study of planktonic and biofilm communities under conditions that are nutritionally reminiscent of the CF airway microenvironment, something not possible using any existing <i>in vivo</i> models of CF airway infection.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2046-1402
dc.sourcenlmid: 101594320
dc.subjectbiofilms
dc.subjectCystic Fibrosis (Cf)
dc.subjectPolymicrobial
dc.subject3Rs
dc.subjectIn Vitro Models
dc.titleAn <i>in vitro</i> model for the cultivation of polymicrobial biofilms under continuous-flow conditions.
dc.typeArticle
dc.date.updated2021-10-26T00:46:20Z
prism.publicationNameF1000Research
prism.volume10
dc.identifier.doi10.17863/CAM.77339
rioxxterms.versionofrecord10.12688/f1000research.55140.1
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidO'Brien, Thomas James [0000-0002-5529-331X]
dc.contributor.orcidHassan, Marwa Mohsen [0000-0001-9629-3802]


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International