Show simple item record

dc.contributor.authorDavis, Lloyd
dc.contributor.authorRadman, Inja
dc.contributor.authorGoutou, Angeliki
dc.contributor.authorTynan, Ailish
dc.contributor.authorBaxter, Kieran
dc.contributor.authorXi, Zhiyan
dc.contributor.authorO'Shea, Jack M
dc.contributor.authorChin, Jason
dc.contributor.authorGreiss, Sebastian
dc.date.accessioned2021-10-26T23:30:27Z
dc.date.available2021-10-26T23:30:27Z
dc.date.issued2021-08-05
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329917
dc.description.abstractSynthetic strategies for optically controlling gene expression may enable the precise spatiotemporal control of genes in any combination of cells that cannot be targeted with specific promoters. We develop an improved genetic code expansion system in Caenorhabditis elegans and use it to create a photoactivatable Cre recombinase. We laser-activate Cre in single neurons within a bilaterally symmetric pair to selectively switch on expression of a loxP-controlled optogenetic channel in the targeted neuron. We use the system to dissect, in freely moving animals, the individual contributions of the mechanosensory neurons PLML/PLMR to the C. elegans touch response circuit, revealing distinct and synergistic roles for these neurons. We thus demonstrate how genetic code expansion and optical targeting can be combined to break the symmetry of neuron pairs and dissect behavioural outputs of individual neurons that cannot be genetically targeted.
dc.format.mediumElectronic
dc.languageeng
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titlePrecise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase.
dc.typeArticle
prism.publicationDate2021
prism.publicationNameElife
prism.volume10
dc.identifier.doi10.17863/CAM.77360
dcterms.dateAccepted2021-08-04
rioxxterms.versionofrecord10.7554/eLife.67075
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-08-05
dc.contributor.orcidO'Shea, Jack M [0000-0002-9694-7340]
dc.contributor.orcidChin, Jason [0000-0003-1219-4757]
dc.contributor.orcidGreiss, Sebastian [0000-0001-9130-0831]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-08-05


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International