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dc.contributor.authorChrysostomou, Andreas C
dc.contributor.authorHezka Rodosthenous, Johana
dc.contributor.authorTopcu, Cicek
dc.contributor.authorPapa, Christina
dc.contributor.authorAristokleous, Antonia
dc.contributor.authorStathi, Georgia
dc.contributor.authorChristodoulou, Christina
dc.contributor.authorEleftheriou, Christina
dc.contributor.authorStylianou, Dora C
dc.contributor.authorKostrikis, Leondios G
dc.date.accessioned2021-10-29T07:14:57Z
dc.date.available2021-10-29T07:14:57Z
dc.date.issued2021-10-27
dc.identifier.issn2075-1729
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330037
dc.descriptionFunder: European Regional Development Fund and the Republic of Cyprus through the Cyprus Research and Innovation Foundation and the Structural Funds 2021-2027; Grant(s): Project: CONCEPT-COVID_0420_0024
dc.description.abstractEmerging infectious viruses have led to global advances in the development of specific and sensitive detection techniques. Viruses have an inherent potential to easily mutate, presenting major hurdles for diagnostics and requiring methods capable of detecting genetically diverse viral strains. One such infectious agent is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in December 2019 and has resulted in the global coronavirus disease 2019 (COVID-19) pandemic. This study presents a real-time reverse transcription PCR (RT-PCR) detection assay for SARS-CoV-2, taking into account its intrinsic polymorphic nature that arises due to genetic drift and recombination, as well as the possibility of continuous and multiple introductions of genetically nonidentical strains into the human population. This advance was achieved by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV-2 S, E, M, and N genes. These were applied to create a simple and reproducible real-time RT-PCR assay, which was validated using external quality control panels (QCMD: CVOP20, WHO: SARS-CoV-2-EQAP-01) and clinical samples. This assay was designed for high target detection accuracy and specificity and can also be readily adapted for the detection of other emerging and rapidly mutating pathogens.
dc.languageen
dc.publisherMDPI AG
dc.subjectCOVID-19
dc.subjectSARS-CoV-2
dc.subjectmolecular beacons
dc.titleA Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2.
dc.typeArticle
dc.date.updated2021-10-29T07:14:56Z
prism.issueIdentifier11
prism.publicationNameLife (Basel)
prism.volume11
dc.identifier.doi10.17863/CAM.77481
dcterms.dateAccepted2021-10-24
rioxxterms.versionofrecord10.3390/life11111146
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidChrysostomou, Andreas C [0000-0002-5796-1588]
dc.contributor.orcidChristodoulou, Christina [0000-0001-6136-4113]
dc.contributor.orcidKostrikis, Leondios G [0000-0002-5340-7109]
dc.identifier.eissn2075-1729
pubs.funder-project-idUniversity of Cyprus (“Research Saves Lives” campaign)
cam.issuedOnline2021-10-27


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