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dc.contributor.authorKontopoulos, Ioannis
dc.contributor.authorPenkman, Kirsty
dc.contributor.authorMullin, Victoria E
dc.contributor.authorWinkelbach, Laura
dc.contributor.authorUnterländer, Martina
dc.contributor.authorScheu, Amelie
dc.contributor.authorKreutzer, Susanne
dc.contributor.authorHansen, Henrik B
dc.contributor.authorMargaryan, Ashot
dc.contributor.authorTeasdale, Matthew
dc.contributor.authorGehlen, Birgit
dc.contributor.authorStreet, Martin
dc.contributor.authorLynnerup, Niels
dc.contributor.authorLiritzis, Ioannis
dc.contributor.authorSampson, Adamantios
dc.contributor.authorPapageorgopoulou, Christina
dc.contributor.authorAllentoft, Morten E
dc.contributor.authorBurger, Joachim
dc.contributor.authorBradley, Daniel G
dc.contributor.authorCollins, Matthew
dc.date.accessioned2021-10-29T23:31:06Z
dc.date.available2021-10-29T23:31:06Z
dc.date.issued2020
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330068
dc.description.abstractThe recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. We establish thresholds for three different FTIR indices, a) the infrared splitting factor [IRSF] that assesses relative changes in bioapatite crystals' size and homogeneity; b) the carbonate-to-phosphate [C/P] ratio as a relative measure of carbonate content in bioapatite crystals; and c) the amide-to-phosphate ratio [Am/P] for assessing the relative organic content preserved in bone. These thresholds are both extremely reliable and easy to apply for the successful and rapid distinction between well- and poorly-preserved specimens. This is a milestone for choosing appropriate samples prior to genomic and collagen analyses, with important implications for biomolecular archaeology and palaeontology.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBone and Bones
dc.subjectAnimals
dc.subjectHumans
dc.subjectSpectroscopy, Fourier Transform Infrared
dc.subjectProteomics
dc.subjectArchaeology
dc.subjectFossils
dc.subjectDNA, Ancient
dc.titleScreening archaeological bone for palaeogenetic and palaeoproteomic studies.
dc.typeArticle
prism.issueIdentifier6
prism.publicationDate2020
prism.publicationNamePLoS One
prism.startingPagee0235146
prism.volume15
dc.identifier.doi10.17863/CAM.77512
dcterms.dateAccepted2020-06-09
rioxxterms.versionofrecord10.1371/journal.pone.0235146
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-01
dc.contributor.orcidKontopoulos, Ioannis [0000-0001-5591-8917]
dc.contributor.orcidKreutzer, Susanne [0000-0001-6286-534X]
dc.contributor.orcidTeasdale, Matthew [0000-0002-7376-9975]
dc.contributor.orcidGehlen, Birgit [0000-0003-1345-8072]
dc.contributor.orcidCollins, Matthew [0000-0003-4226-5501]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Review
cam.issuedOnline2020-06-25


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International