Identification of GOLPH3 Partners in <i>Drosophila</i> Unveils Potential Novel Roles in Tumorigenesis and Neural Disorders.
Di Francesco, Laura
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Sechi, S., Karimpour-Ghahnavieh, A., Frappaolo, A., Di Francesco, L., Piergentili, R., Schininà, E., D'Avino, P. P., & et al. (2021). Identification of GOLPH3 Partners in <i>Drosophila</i> Unveils Potential Novel Roles in Tumorigenesis and Neural Disorders.. Cells, 10 (9) https://doi.org/10.3390/cells10092336
Golgi phosphoprotein 3 (GOLPH3) is a highly conserved peripheral membrane protein localized to the Golgi apparatus and the cytosol. GOLPH3 binding to Golgi membranes depends on phosphatidylinositol 4-phosphate [PI(4)P] and regulates Golgi architecture and vesicle trafficking. GOLPH3 overexpression has been correlated with poor prognosis in several cancers, but the molecular mechanisms that link GOLPH3 to malignant transformation are poorly understood. We recently showed that PI(4)P-GOLPH3 couples membrane trafficking with contractile ring assembly during cytokinesis in dividing <i>Drosophila</i> spermatocytes. Here, we use affinity purification coupled with mass spectrometry (AP-MS) to identify the protein-protein interaction network (interactome) of <i>Drosophila</i> GOLPH3 in testes. Analysis of the GOLPH3 interactome revealed enrichment for proteins involved in vesicle-mediated trafficking, cell proliferation and cytoskeleton dynamics. In particular, we found that dGOLPH3 interacts with the <i>Drosophila</i> orthologs of Fragile X mental retardation protein and Ataxin-2, suggesting a potential role in the pathophysiology of disorders of the nervous system. Our findings suggest novel molecular targets associated with GOLPH3 that might be relevant for therapeutic intervention in cancers and other human diseases.
Spermatogenesis, Drosophila, Cell cycle, FMRP, Golgi, Golph3, Male Meiosis
Royal Society International Exchanges Award (2016/R2 International Exchanges IE160510)
Fondazione Italiana per la Ricerca sul Cancro (id. 19686)
Associazione Italiana per la Ricerca sul Cancro (IG2017 Id.20779)
External DOI: https://doi.org/10.3390/cells10092336
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330097
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/