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dc.contributor.authorPanda, Pallavi
dc.contributor.authorKovacs, Levente
dc.contributor.authorDzhindzhev, Nikola
dc.contributor.authorFatalska, Agnieszka
dc.contributor.authorPersico, Veronica
dc.contributor.authorGeymonat, Marco
dc.contributor.authorRiparbelli, Maria Giovanna
dc.contributor.authorCallaini, Giuliano
dc.contributor.authorGlover, David M
dc.date.accessioned2021-11-04T00:30:47Z
dc.date.available2021-11-04T00:30:47Z
dc.date.issued2020-09-07
dc.identifier.issn0021-9525
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330263
dc.description.abstractRcd4 is a poorly characterized Drosophila centriole component whose mammalian counterpart, PPP1R35, is suggested to function in centriole elongation and conversion to centrosomes. Here, we show that rcd4 mutants exhibit fewer centrioles, aberrant mitoses, and reduced basal bodies in sensory organs. Rcd4 interacts with the C-terminal part of Ana3, which loads onto the procentriole during interphase, ahead of Rcd4 and before mitosis. Accordingly, depletion of Ana3 prevents Rcd4 recruitment but not vice versa. We find that neither Ana3 nor Rcd4 participates directly in the mitotic conversion of centrioles to centrosomes, but both are required to load Ana1, which is essential for such conversion. Whereas ana3 mutants are male sterile, reflecting a requirement for Ana3 for centriole development in the male germ line, rcd4 mutants are fertile and have male germ line centrioles of normal length. Thus, Rcd4 is essential in somatic cells but is not absolutely required in spermatogenesis, indicating tissue-specific roles in centriole and basal body formation.
dc.languageeng
dc.publisherRockefeller University Press
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectAxoneme
dc.subjectCentrioles
dc.subjectCilia
dc.subjectDrosophila Proteins
dc.subjectFemale
dc.subjectMale
dc.subjectMutation
dc.subjectOrganelle Biogenesis
dc.subjectProtein Binding
dc.subjectSpermatogenesis
dc.titleTissue specific requirement of Drosophila Rcd4 for centriole duplication and ciliogenesis.
dc.typeArticle
prism.issueIdentifier8
prism.numbere201912154
prism.publicationDate2020
prism.publicationNameThe Journal of Cell Biology
prism.volume219
dc.identifier.doi10.17863/CAM.77704
dcterms.dateAccepted2020-05-13
rioxxterms.versionofrecord10.1083/jcb.201912154
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2020-09-07
dc.contributor.orcidPanda, Pallavi [0000-0001-7572-6728]
dc.contributor.orcidGeymonat, Marco [0000-0002-8792-0517]
dc.contributor.orcidGlover, David [0000-0003-0956-0103]
dc.identifier.eissn1540-8140
rioxxterms.typeJournal Article/Review
cam.issuedOnline2020-06-16


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International