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dc.contributor.authorRomanos, Michèle
dc.contributor.authorAllio, Guillaume
dc.contributor.authorRoussigné, Myriam
dc.contributor.authorCombres, Léa
dc.contributor.authorEscalas, Nathalie
dc.contributor.authorSoula, Cathy
dc.contributor.authorMédevielle, François
dc.contributor.authorSteventon, Benjamin
dc.contributor.authorTrescases, Ariane
dc.contributor.authorBénazéraf, Bertrand
dc.date.accessioned2021-11-06T02:06:58Z
dc.date.available2021-11-06T02:06:58Z
dc.date.issued2021-10-05
dc.identifier.issn2050-084X
dc.identifier.otherPMC8492064
dc.identifier.other34607629
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330394
dc.description.abstractAlthough cell-to-cell heterogeneity in gene and protein expression within cell populations has been widely documented, we know little about its biological functions. By studying progenitors of the posterior region of bird embryos, we found that expression levels of transcription factors Sox2 and Bra, respectively involved in neural tube (NT) and mesoderm specification, display a high degree of cell-to-cell heterogeneity. By combining forced expression and downregulation approaches with time-lapse imaging, we demonstrate that Sox2-to-Bra ratio guides progenitor's motility and their ability to stay in or exit the progenitor zone to integrate neural or mesodermal tissues. Indeed, high Bra levels confer high motility that pushes cells to join the paraxial mesoderm, while high levels of Sox2 tend to inhibit cell movement forcing cells to integrate the NT. Mathematical modeling captures the importance of cell motility regulation in this process and further suggests that randomness in Sox2/Bra cell-to-cell distribution favors cell rearrangements and tissue shape conservation.
dc.languageeng
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2050-084X
dc.sourcenlmid: 101579614
dc.subjectMorphogenesis
dc.subjectCell motility
dc.subjectHeterogeneity
dc.subjectdevelopmental biology
dc.subjectMathematical Modeling
dc.subjectNeuromesodermal Progenitors
dc.subjectBird Embryos
dc.titleCell-to-cell heterogeneity in Sox2 and Bra expression guides progenitor motility and destiny.
dc.typeArticle
dc.date.updated2021-11-06T02:06:57Z
prism.publicationNameElife
prism.volume10
dc.identifier.doi10.17863/CAM.77837
dcterms.dateAccepted2021-09-13
rioxxterms.versionofrecord10.7554/eLife.66588
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidRoussigné, Myriam [0000-0002-4240-4105]
dc.contributor.orcidSteventon, Benjamin [0000-0001-7838-839X]
dc.contributor.orcidBénazéraf, Bertrand [0000-0002-1937-637X]
dc.identifier.eissn2050-084X
pubs.funder-project-idFondation ARC pour la Recherche sur le Cancer (PJA 20191209486)
pubs.funder-project-idAgence Nationale de la Recherche (MULTIEL ANRJC)
cam.issuedOnline2021-10-05


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International