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dc.contributor.authorOeller, Marc
dc.contributor.authorSormanni, Pietro
dc.contributor.authorVendruscolo, Michele
dc.date.accessioned2021-11-09T16:34:07Z
dc.date.available2021-11-09T16:34:07Z
dc.date.issued2021-11-09
dc.date.submitted2021-07-30
dc.identifier.others41598-021-01126-4
dc.identifier.other1126
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330496
dc.description.abstractAbstract: The solubility of proteins correlates with a variety of their properties, including function, production yield, pharmacokinetics, and formulation at high concentrations. High solubility is therefore a key requirement for the development of protein-based reagents for applications in life sciences, biotechnology, diagnostics, and therapeutics. Accurate solubility measurements, however, remain challenging and resource intensive, which limits their throughput and hence their applicability at the early stages of development pipelines, when long-lists of candidates are typically available in minute amounts. Here, we present an automated method based on the titration of a crowding agent (polyethylene glycol, PEG) to quantitatively assess relative solubility of proteins using about 200 µg of purified material. Our results demonstrate that this method is accurate and economical in material requirement and costs of reagents, which makes it suitable for high-throughput screening. This approach is freely-shared and based on a low cost, open-source liquid-handling robot. We anticipate that this method will facilitate the assessment of the developability of proteins and make it substantially more accessible.
dc.languageen
dc.publisherNature Publishing Group UK
dc.subjectArticle
dc.subject/631/1647/2196
dc.subject/631/1647/2204
dc.subjectarticle
dc.titleAn open-source automated PEG precipitation assay to measure the relative solubility of proteins with low material requirement
dc.typeArticle
dc.date.updated2021-11-09T16:34:07Z
prism.issueIdentifier1
prism.publicationNameScientific Reports
prism.volume11
dc.identifier.doi10.17863/CAM.77939
dcterms.dateAccepted2021-10-18
rioxxterms.versionofrecord10.1038/s41598-021-01126-4
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2045-2322


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