Controlled In-Cell Generation of Active Palladium(0) Species for Bioorthogonal Decaging.
Pérez, Laura Rodríguez
Domingos, Josiel B
Angewandte Chemie International Edition
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Konč, J., Sabatino, V., Jiménez-Moreno, E., Latocheski, E., Pérez, L. R., Day, J., Domingos, J. B., & et al. (2022). Controlled In-Cell Generation of Active Palladium(0) Species for Bioorthogonal Decaging.. Angewandte Chemie International Edition https://doi.org/10.1002/anie.202113519
Owing to their bioorthogonality, transition metals have become very popular in the development of biocompatible bond-cleavage reactions. However, many approaches require design and synthesis of complex ligands or formulation of nanoparticles which often perform poorly in living cells. This work reports on a method for the generation of an active palladium species that triggers bond-cleaving reactions inside living cells. We utilized the water-soluble Na2PdCl4 as a simple source of Pd(II) which can be intracellularly reduced by sodium ascorbate to the active Pd(0) species. Once generated, Pd(0) triggers the cleavage of allyl ether and carbamate caging groups leading to the release of biologically active molecules. These findings do not only expand the toolbox of available bioorthogonal dissociative reactions but also provide an additional strategy for controlling the reactivity of Pd species involved in Pd-mediated bioorthogonal reactions.
Cancer, Palladium, bioorthogonal, bond-cleavage, decaging
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (701473)
European Research Council (676832)
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External DOI: https://doi.org/10.1002/anie.202113519
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330524
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