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Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Fazakerley, Daniel J 
Fritzen, Andreas M 
Nelson, Marin E 
Thorius, Ida H 
Cooke, Kristen C 

Abstract

Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

Description

Keywords

Adipose tissue, Glucose transport, Glucose uptake, Insulin, Insulin tolerance test, Muscle

Journal Title

Bio Protoc

Conference Name

Journal ISSN

2331-8325
2331-8325

Volume Title

9

Publisher

Bio-Protocol, LLC