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dc.contributor.authorCheng, Jinmei
dc.contributor.authorAllgeyer, Edward
dc.contributor.authorRichens, Jennifer
dc.contributor.authorDzafic, Edo
dc.contributor.authorPalandri, Amandine
dc.contributor.authorLewków, Bohdan
dc.contributor.authorSirinakis, George
dc.contributor.authorSt Johnston, Daniel
dc.date.accessioned2021-11-17T00:31:07Z
dc.date.available2021-11-17T00:31:07Z
dc.date.issued2021-12-15
dc.identifier.issn0021-9533
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330699
dc.description.abstractSingle-molecule localization microscopy (SMLM) can provide nanoscale resolution in thin samples but has rarely been applied to tissues because of high background from out-of-focus emitters and optical aberrations. Here, we describe a line scanning microscope that provides optical sectioning for SMLM in tissues. Imaging endogenously-tagged nucleoporins and F-actin on this system using DNA- and peptide-point accumulation for imaging in nanoscale topography (PAINT) routinely gives 30 nm resolution or better at depths greater than 20 µm. This revealed that the nuclear pores are nonrandomly distributed in most Drosophila tissues, in contrast to what is seen in cultured cells. Lamin Dm0 shows a complementary localization to the nuclear pores, suggesting that it corrals the pores. Furthermore, ectopic expression of the tissue-specific Lamin C causes the nuclear pores to distribute more randomly, whereas lamin C mutants enhance nuclear pore clustering, particularly in muscle nuclei. Given that nucleoporins interact with specific chromatin domains, nuclear pore clustering could regulate local chromatin organization and contribute to the disease phenotypes caused by human lamin A/C laminopathies.
dc.description.sponsorshipJiangsu Government Scholarship for Overseas Studies, China to Jinmei Cheng
dc.publisherThe Company of Biologists
dc.rightsAll rights reserved
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.titleA single-molecule localization microscopy method for tissues reveals nonrandom nuclear pore distribution in Drosophila.
dc.typeArticle
prism.publicationNameJ Cell Sci
dc.identifier.doi10.17863/CAM.78144
dcterms.dateAccepted2021-11-11
rioxxterms.versionofrecord10.1242/jcs.259570
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-11-11
dc.contributor.orcidCheng, Jinmei [0000-0002-2824-6892]
dc.contributor.orcidAllgeyer, Edward [0000-0002-2187-4423]
dc.contributor.orcidRichens, Jennifer [0000-0002-8241-4826]
dc.contributor.orcidDzafic, Edo [0000-0002-7155-4911]
dc.contributor.orcidPalandri, Amandine [0000-0002-2472-4498]
dc.contributor.orcidSirinakis, George [0000-0002-4762-422X]
dc.contributor.orcidSt Johnston, Daniel [0000-0001-5582-3301]
dc.identifier.eissn1477-9137
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idWellcome Trust (203144/Z/16/Z)
pubs.funder-project-idWellcome Trust (203285/Z/16/Z)
pubs.funder-project-idWellcome Trust (080007/Z/06/Z)
pubs.funder-project-idWellcome Trust (080007/B/06/Z)
pubs.funder-project-idWellcome Trust (109143/Z/15/Z)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/P026486/1)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idCancer Research UK (C6946/A24843)
pubs.funder-project-idWellcome Trust (095927/B/11/Z)
cam.issuedOnline2021-12-16
cam.orpheus.successTue Feb 01 19:02:16 GMT 2022 - Embargo updated*
cam.orpheus.counter1
rioxxterms.freetoread.startdate2022-12-15


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