Structural basis for the interaction of SARS-CoV-2 virulence factor nsp1 with DNA polymerase α-primase.
Authors
Veale, Charlotte E
Guppy, Amir
Rzechorzek, Neil J
Maman, Joseph
Publication Date
2022-02Journal Title
Protein Sci
ISSN
0961-8368
Publisher
Wiley
Language
en
Type
Article
This Version
AO
VoR
Metadata
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Kilkenny, M., Veale, C. E., Guppy, A., Hardwick, S., Chirgadze, D., Rzechorzek, N. J., Maman, J., & et al. (2022). Structural basis for the interaction of SARS-CoV-2 virulence factor nsp1 with DNA polymerase α-primase.. Protein Sci https://doi.org/10.1002/pro.4220
Abstract
The molecular mechanisms that drive the infection by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-the causative agent of coronavirus disease 2019 (COVID-19)-are under intense current scrutiny to understand how the virus operates and to uncover ways in which the disease can be prevented or alleviated. Recent proteomic screens of the interactions between viral and host proteins have identified the human proteins targeted by SARS-CoV-2. The DNA polymerase α (Pol α)-primase complex or primosome-responsible for initiating DNA synthesis during genomic duplication-was identified as a target of nonstructural protein 1 (nsp1), a major virulence factor in the SARS-CoV-2 infection. Here, we validate the published reports of the interaction of nsp1 with the primosome by demonstrating direct binding with purified recombinant components and providing a biochemical characterization of their interaction. Furthermore, we provide a structural basis for the interaction by elucidating the cryo-electron microscopy structure of nsp1 bound to the primosome. Our findings provide biochemical evidence for the reported targeting of Pol α by the virulence factor nsp1 and suggest that SARS-CoV-2 interferes with Pol α's putative role in the immune response during the viral infection.
Keywords
Full‐Length Paper, Full‐Length Papers, cryo‐EM, DNA polymerase α–primase, nsp1, protein–protein interaction, SARS‐CoV‐2
Sponsorship
Wellcome Trust (104641/Z/14/Z)
Identifiers
pro4220
External DOI: https://doi.org/10.1002/pro.4220
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330770
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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