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dc.contributor.authorKeller, Christian
dc.contributor.authorRakotozandrindrainy, Raphaël
dc.contributor.authorvon Kalckreuth, Vera
dc.contributor.authorHeriniaina, Jean Noël
dc.contributor.authorSchwarz, Norbert Georg
dc.contributor.authorPak, Gi Deok
dc.contributor.authorIm, Justin
dc.contributor.authorEspinoza, Ligia Maria Cruz
dc.contributor.authorHagen, Ralf Matthias
dc.contributor.authorFrickmann, Hagen
dc.contributor.authorRakotondrainiarivelo, Jean Philibert
dc.contributor.authorRazafindrabe, Tsiry
dc.contributor.authorDekker, Denise
dc.contributor.authorMay, Jürgen
dc.contributor.authorPoppert, Sven
dc.contributor.authorMarks, Florian
dc.date.accessioned2021-11-22T14:38:48Z
dc.date.available2021-11-22T14:38:48Z
dc.date.issued2021-11-14
dc.identifier.issn2076-0817
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330829
dc.description.abstractRickettsiae may cause febrile infections in humans in tropical and subtropical regions. From Madagascar, no molecular data on the role of rickettsioses in febrile patients are available. Blood samples from patients presenting with fever in the area of the capital Antananarivo were screened for the presence of rickettsial DNA. EDTA (ethylenediaminetetraacetic acid) blood from 1020 patients presenting with pyrexia > 38.5 °C was analyzed by gltA-specific qPCR. Positive samples were confirmed by ompB-specific qPCR. From confirmed samples, the gltA amplicons were sequenced and subjected to phylogenetic analysis. From five gltA-reactive samples, two were confirmed by ompB-specific qPCR. The gltA sequence in the sample taken from a 38-year-old female showed 100% homology with R. typhi. The other sample taken from a 1.5-year-old infant was 100% homologous to R. felis. Tick-borne rickettsiae were not identified. The overall rate of febrile patients with molecular evidence for a rickettsial infection from the Madagascan study site was 0.2% (2/1020 patients). Flea-borne rickettsiosis is a rare but neglected cause of infection in Madagascar. Accurate diagnosis may prompt adequate antimicrobial treatment.
dc.languageen
dc.publisherMDPI AG
dc.subjectRickettsia typhi
dc.subjectRickettsia felis
dc.subjectMadagascar
dc.subjectflea-borne rickettsiosis
dc.subjectmurine typhus
dc.titleMolecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar.
dc.typeArticle
dc.date.updated2021-11-22T14:38:48Z
prism.issueIdentifier11
prism.publicationNamePathogens
prism.volume10
dc.identifier.doi10.17863/CAM.78272
dcterms.dateAccepted2021-11-09
rioxxterms.versionofrecord10.3390/pathogens10111482
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidKeller, Christian [0000-0002-0075-7199]
dc.contributor.orcidHagen, Ralf Matthias [0000-0003-4875-1519]
dc.contributor.orcidFrickmann, Hagen [0000-0002-8967-9528]
dc.contributor.orcidDekker, Denise [0000-0001-5432-3217]
dc.contributor.orcidPoppert, Sven [0000-0003-4873-249X]
dc.contributor.orcidMarks, Florian [0000-0002-6043-7170]
dc.identifier.eissn2076-0817
pubs.funder-project-idBill &amp (OPPGH5231)
cam.issuedOnline2021-11-14


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