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dc.contributor.authorJarvis, Lorna B
dc.contributor.authorRainbow, Daniel B
dc.contributor.authorCoppard, Valerie
dc.contributor.authorHowlett, Sarah K
dc.contributor.authorGeorgieva, Zoya
dc.contributor.authorDavies, Jessica L
dc.contributor.authorMullay, Harpreet Kaur
dc.contributor.authorHester, Joanna
dc.contributor.authorAshmore, Tom
dc.contributor.authorVan Den Bosch, Aletta
dc.contributor.authorGrist, James T
dc.contributor.authorColes, Alasdair J
dc.contributor.authorMousa, Hani S
dc.contributor.authorPluchino, Stefano
dc.contributor.authorMahbubani, Krishnaa T
dc.contributor.authorGriffin, Julian L
dc.contributor.authorSaeb-Parsy, Kourosh
dc.contributor.authorIssa, Fadi
dc.contributor.authorPeruzzotti-Jametti, Luca
dc.contributor.authorWicker, Linda S
dc.contributor.authorJones, Joanne L
dc.date.accessioned2021-11-22T14:39:08Z
dc.date.available2021-11-22T14:39:08Z
dc.date.issued2021-10-14
dc.identifier.issn2399-3642
dc.identifier.otherPMC8516976
dc.identifier.other34650224
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330831
dc.description.abstractThe adoptive transfer of regulatory T-cells (Tregs) is a promising therapeutic approach in transplantation and autoimmunity. However, because large cell numbers are needed to achieve a therapeutic effect, in vitro expansion is required. By comparing their function, phenotype and transcriptomic profile against ex vivo Tregs, we demonstrate that expanded human Tregs switch their metabolism to aerobic glycolysis and show enhanced suppressive function through hypoxia-inducible factor 1-alpha (HIF1A) driven acquisition of CD73 expression. In conjunction with CD39, CD73 expression enables expanded Tregs to convert ATP to immunosuppressive adenosine. We conclude that for maximum therapeutic benefit, Treg expansion protocols should be optimised for CD39/CD73 co-expression.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2399-3642
dc.sourcenlmid: 101719179
dc.titleTherapeutically expanded human regulatory T-cells are super-suppressive due to HIF1A induced expression of CD73.
dc.typeArticle
dc.date.updated2021-11-22T14:39:07Z
prism.issueIdentifier1
prism.publicationNameCommunications biology
prism.volume4
dc.identifier.doi10.17863/CAM.78274
rioxxterms.versionofrecord10.1038/s42003-021-02721-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidJarvis, Lorna B [0000-0002-5760-0125]
dc.contributor.orcidRainbow, Daniel B [0000-0003-4931-3289]
dc.contributor.orcidDavies, Jessica L [0000-0002-8888-1441]
dc.contributor.orcidHester, Joanna [0000-0002-7466-3849]
dc.contributor.orcidMousa, Hani S [0000-0002-8327-7114]
dc.contributor.orcidPluchino, Stefano [0000-0002-6267-9472]
dc.contributor.orcidMahbubani, Krishnaa T [0000-0002-1327-2334]
dc.contributor.orcidGriffin, Julian L [0000-0003-1336-7744]
dc.contributor.orcidSaeb-Parsy, Kourosh [0000-0002-0633-3696]
dc.contributor.orcidIssa, Fadi [0000-0002-8279-7732]
dc.contributor.orcidWicker, Linda S [0000-0001-7771-0324]
dc.contributor.orcidJones, Joanne L [0000-0003-4974-1371]
pubs.funder-project-idWellcome Trust (RG79413, 105924/Z/14/Z, 220554/Z/20/Z)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International