Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development.
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Authors
Sankaranarayanan, M
Emenecker, Ryan J
Wilby, Elise L
Jahnel, Marcus
Trussina, Irmela REA
Alberti, Simon
Holehouse, Alex S
Publication Date
2021-10-25Journal Title
Dev Cell
ISSN
1534-5807
Publisher
Elsevier BV
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Sankaranarayanan, M., Emenecker, R. J., Wilby, E. L., Jahnel, M., Trussina, I. R., Wayland, M., Alberti, S., et al. (2021). Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development.. Dev Cell https://doi.org/10.1016/j.devcel.2021.09.021
Abstract
Ribonucleoprotein condensates can exhibit diverse physical states in vitro and in vivo. Despite considerable progress, the relevance of condensate physical states for in vivo biological function remains limited. Here, we investigated the physical properties of processing bodies (P bodies) and their impact on mRNA storage in mature Drosophila oocytes. We show that the conserved DEAD-box RNA helicase Me31B forms viscous P body condensates, which adopt an arrested physical state. We demonstrate that structurally distinct proteins and protein-protein interactions, together with RNA, regulate the physical properties of P bodies. Using live imaging and in situ hybridization, we show that the arrested state and integrity of P bodies support the storage of bicoid (bcd) mRNA and that egg activation modulates P body properties, leading to the release of bcd for translation in the early embryo. Together, this work provides an example of how physical states of condensates regulate cellular function in development.
Keywords
Drosophila, Ribonucleoprotein, Phase separation, Mrna Regulation, Axis Patterning, Processing Bodies, In vivo Imaging, Intrinsically Disordered Regions, Biomolecular Condensates
Sponsorship
Wellcome Trust (200734/Z/16/Z)
Identifiers
PMC8555633, 34655524
External DOI: https://doi.org/10.1016/j.devcel.2021.09.021
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330850
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