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dc.contributor.authorSankaranarayanan, M
dc.contributor.authorEmenecker, Ryan J
dc.contributor.authorWilby, Elise L
dc.contributor.authorJahnel, Marcus
dc.contributor.authorTrussina, Irmela REA
dc.contributor.authorWayland, Matthew
dc.contributor.authorAlberti, Simon
dc.contributor.authorHolehouse, Alex S
dc.contributor.authorWeil, Timothy
dc.date.accessioned2021-11-22T14:41:54Z
dc.date.available2021-11-22T14:41:54Z
dc.date.issued2021-10-25
dc.identifier.issn1534-5807
dc.identifier.otherPMC8555633
dc.identifier.other34655524
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330850
dc.description.abstractRibonucleoprotein condensates can exhibit diverse physical states in vitro and in vivo. Despite considerable progress, the relevance of condensate physical states for in vivo biological function remains limited. Here, we investigated the physical properties of processing bodies (P bodies) and their impact on mRNA storage in mature Drosophila oocytes. We show that the conserved DEAD-box RNA helicase Me31B forms viscous P body condensates, which adopt an arrested physical state. We demonstrate that structurally distinct proteins and protein-protein interactions, together with RNA, regulate the physical properties of P bodies. Using live imaging and in situ hybridization, we show that the arrested state and integrity of P bodies support the storage of bicoid (bcd) mRNA and that egg activation modulates P body properties, leading to the release of bcd for translation in the early embryo. Together, this work provides an example of how physical states of condensates regulate cellular function in development.
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1878-1551
dc.sourcenlmid: 101120028
dc.subjectDrosophila
dc.subjectRibonucleoprotein
dc.subjectPhase separation
dc.subjectMrna Regulation
dc.subjectAxis Patterning
dc.subjectProcessing Bodies
dc.subjectIn vivo Imaging
dc.subjectIntrinsically Disordered Regions
dc.subjectBiomolecular Condensates
dc.titleAdaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development.
dc.typeArticle
dc.date.updated2021-11-22T14:41:53Z
prism.publicationNameDev Cell
dc.identifier.doi10.17863/CAM.78293
dcterms.dateAccepted2021-09-23
rioxxterms.versionofrecord10.1016/j.devcel.2021.09.021
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidWayland, Matthew [0000-0002-8095-858X]
dc.contributor.orcidWeil, Timothy [0000-0003-0490-849X]
dc.identifier.eissn1878-1551
pubs.funder-project-idWellcome Trust (200734/Z/16/Z)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International