A Platform for Site‐Specific DNA‐Antibody Bioconjugation by Using Benzoylacrylic‐Labelled Oligonucleotides
Authors
Konč, Juraj
Brown, Libby
Whiten, Daniel R
Zuo, Yukun
Publication Date
2021-12Journal Title
Angewandte Chemie
ISSN
0044-8249
Publisher
Wiley
Volume
133
Issue
49
Pages
26109-26117
Language
en
Type
Article
This Version
AO
VoR
Metadata
Show full item recordCitation
Konč, J., Brown, L., Whiten, D. R., Zuo, Y., Ravn, P., Klenerman, D., & Lopes Bernardes, G. (2021). A Platform for Site‐Specific DNA‐Antibody Bioconjugation by Using Benzoylacrylic‐Labelled Oligonucleotides. Angewandte Chemie, 133 (49), 26109-26117. https://doi.org/10.1002/ange.202109713
Abstract
Many bioconjugation strategies for DNA oligonucleotides and antibodies suffer limitations, such as site-specificity, stoichiometry and hydrolytic instability of the conjugates, which makes them unsuitable for biological applications. Here, we report a new platform for the preparation of DNA-antibody bioconjugates with a simple benzoylacrylic acid pentafluorophenyl ester reagent. Benzoylacrylic-labelled oligonucleotides prepared with this reagent can be site-specifically conjugated to a range of proteins and antibodies through accessible cysteine residues. The homogeneity of the prepared DNA-antibody bioconjugates was confirmed by a new LC-MS protocol and the bioconjugate probes were used in fluorescence or super-resolution microscopy cell imaging experiments. This work demonstrates the versatility and robustness of our bioconjugation protocol that gives site-specific, well-defined and plasma-stable DNA-antibody bioconjugates for biological applications.
Keywords
Forschungsartikel, antibodies, bioconjugation, imaging, mass spectrometry, nucleic acids
Sponsorship
European Research Council (676832)
European Commission (EC) (852985)
Identifiers
ange202109713
External DOI: https://doi.org/10.1002/ange.202109713
This record's URL: https://www.repository.cam.ac.uk/handle/1810/331023
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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